Name: Anti-TMEFF2 Antibody (CAB10464)
Brand: Cell Biology Antibodies 1
SKU: CAB10464
Availability: OutOfStock

Antibody Name:	Anti-TMEFF2 Antibody
Antibody SKU:	CAB10464
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 41-320 of human TMEFF2 (NP_057276.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, A-549, SW480, HeLa, Mouse brain, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 41-320 of human TMEFF2 (NP_057276.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FPTS LSDC QTPT GWNC SGYD DREN DLFL CDTN TCKF DGEC LRIG DTVT CVCQ FKCN NDYV PVCG SNGE SYQN ECYL RQAA CKQQ SEIL VVSE GSCA TDAG SGSG DGVH EGSG ETSQ KETS TCDI CQFG AECD EDAE DVWC VCNI DCSQ TNFN PLCA SDGK SYDN ACQI KEAS CQKQ EKIE VMSL GRCQ DNTT TTTK SEDG HYAR TDYA ENAN KLEE SARE HHIP CPEH YNGF CMHG KCEH SINM QEPS CRCD AGYT GQHC EKKD YSVL YVVP GPVR FQYV
Gene ID:	23671
Uniprot:	Q9UIK5
Cellular Location:	Membrane, Secreted, Single-pass type I membrane protein, Single-pass type I membrane protein
Calculated MW:	19kDa/38kDa/41kDa
Observed MW:	41kDa

Synonyms:

TMEFF2, CT120.2, HPP1, TENB2, TPEF, TR, TR-2

Background:

This gene encodes a member of the tomoregulin family of transmembrane proteins. This protein has been shown to function as both an oncogene and a tumor suppressor depending on the cellular context and may regulate prostate cancer cell invasion. Multiple soluble forms of this protein have been identified that arise from both an alternative splice variant and ectodomain shedding. Additionally, this gene has been found to be hypermethylated in multiple cancer types. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	TMEFF2: may be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation. A member of the Cancer-Testis Antigen (CTA) superfamily. CTAs may play roles in embryonal development and tumor transformation or aspects of tumor progression. CTAs were once thought to be silenced in most normal adult tissues, with limited expression in fetal, placental, testis, and ovarian cells. These proteins are now known to be aberrantly expressed in various cancers and many are capable of eliciting humoral and cellular immune responses. Highly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques. Down-regulated in tumor cell lines in response to a high level of methylation in the 5' region. The CpG island methylation correlates with TMEFF2 silencing in tumor cell lines. Belongs to the tomoregulin family. Three isoforms of the human protein are produced by alternative splicing. Isoform three is expressed weakly in testis and at high levels in normal and cancerous prostate. Note: This description may include information from RefSeq and UniProtKB
UniProt Protein Details:	Protein type:Cancer Testis Antigen (CTA); Cell surface; Membrane protein, integral Chromosomal Location of Human Ortholog: 2q32.3 Biological Process: negative regulation of cell migration; negative regulation of integrin biosynthetic process; negative regulation of stress fiber formation
NCBI Summary:	This gene encodes a member of the tomoregulin family of transmembrane proteins. This protein has been shown to function as both an oncogene and a tumor suppressor depending on the cellular context and may regulate prostate cancer cell invasion. Multiple soluble forms of this protein have been identified that arise from both an alternative splice variant and ectodomain shedding. Additionally, this gene has been found to be hypermethylated in multiple cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
UniProt Code:	Q9UIK5
NCBI GenInfo Identifier:	71153590
NCBI Gene ID:	23671
NCBI Accession:	Q9UIK5.1
UniProt Secondary Accession:	Q9UIK5,Q2FA44, Q4ZFW4, Q53H90, Q53RE1, Q8N2R5, Q9NR15 Q9NSS5, Q9P2Y9, Q9UK65,
UniProt Related Accession:	Q9UIK5
Molecular Weight:	41kDa
NCBI Full Name:	Tomoregulin-2
NCBI Synonym Full Names:	transmembrane protein with EGF like and two follistatin like domains 2
NCBI Official Symbol:	TMEFF2
NCBI Official Synonym Symbols:	TR; HPP1; TPEF; TR-2; TENB2; CT120.2
NCBI Protein Information:	tomoregulin-2
UniProt Protein Name:	Tomoregulin-2
UniProt Synonym Protein Names:	Hyperplastic polyposis protein 1; Transmembrane protein with EGF-like and two follistatin-like domains
Protein Family:	Tomoregulin
UniProt Gene Name:	TMEFF2

Western blot analysis of extracts of various cell lines, using TMEFF2 antibody (CAB10464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

TMEFF2 Antibody (PACO12791)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)