Immunology Antibodies 1
Anti-TIRAP Antibody (CAB12606)
- SKU:
- CAB12606
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-TIRAP Antibody |
| Antibody SKU: | CAB12606 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human TIRAP (NP_001034750.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human TIRAP (NP_001034750.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASS TSLP APGS RPKK PLGK MADW FRQT LLKK PKKR PNSP ESTS SDAS QPTS QDSP LPPS LSSV TSPS LPPT HASD SGSS RWSK DYDV CVCH SEED LVAA QDLV SYLE GSTA SLRC FLQL RDAT PGGA IVSE LCQA LSSS HCRV LLIT PGFL QDPW CKYQ MLQA LTEA PGAE GCTI PLLS GLSR AAYP PELR FMYY VDGR GPDG GFRQ VKEA VMRY LQTL S |
| Gene ID: | 114609 |
| Uniprot: | P58753 |
| Cellular Location: | Cell membrane, Cytoplasm, Membrane |
| Calculated MW: | 23kDa/25kDa/27kDa |
| Observed MW: | 32kDa |
| Synonyms: | TIRAP, BACTS1, Mal, MyD88-2, wyatt |
| Background: | The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. |
| UniProt Protein Function: | TIRAP: an adapter protein involved in the TLR4 signaling pathway in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, ERK1, ERK2 and JNK, resulting in cytokine secretion and the inflammatory response. Homodimerizes. Also forms heterodimers with MyD88. Binds to TLR4 and IRAK2 via their respective TIR domains. Binds to PKR and TBK1. Does not interact with IRAK1, nor TLR9. Genetic variations in TIRAP can influence susceptibility or resistance to infectious diseases, including invasive pneumococcal disease, malaria and tuberculosis. Three isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 11q24.2 Cellular Component: nucleoplasm; intercellular bridge; endocytic vesicle; cytoplasm; plasma membrane; cytosol Molecular Function:protein binding, bridging; protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein homodimerization activity; protein kinase C binding; protein heterodimerization activity Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; response to lipopolysaccharide; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; cell surface receptor linked signal transduction; positive regulation of interleukin-15 production; positive regulation of B cell proliferation; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of toll-like receptor 3 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor 2 signaling pathway; regulation of innate immune response; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; toll-like receptor signaling pathway; innate immune response; myeloid cell differentiation; regulation of interferon-beta production Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Bacteremia, Susceptibility To, 1; Mycobacterium Tuberculosis, Susceptibility To; Malaria, Susceptibility To |
| NCBI Summary: | The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P58753 |
| NCBI GenInfo Identifier: | 50403750 |
| NCBI Gene ID: | 114609 |
| NCBI Accession: | P58753.2 |
| UniProt Secondary Accession: | P58753,Q56UH9, Q56UI0, Q8N5E5, B3KW65, |
| UniProt Related Accession: | P58753 |
| Molecular Weight: | 221 |
| NCBI Full Name: | Toll/interleukin-1 receptor domain-containing adapter protein |
| NCBI Synonym Full Names: | toll-interleukin 1 receptor (TIR) domain containing adaptor protein |
| NCBI Official Symbol: | TIRAP |
| NCBI Official Synonym Symbols: | Mal; wyatt; BACTS1; MyD88-2 |
| NCBI Protein Information: | toll/interleukin-1 receptor domain-containing adapter protein; adapter protein wyatt; adaptor protein Wyatt; MyD88 adapter-like protein; Toll-like receptor adaptor protein |
| UniProt Protein Name: | Toll/interleukin-1 receptor domain-containing adapter protein |
| UniProt Synonym Protein Names: | Adaptor protein Wyatt; MyD88 adapter-like protein; MyD88-2 |
| Protein Family: | Toll/interleukin-1 receptor domain-containing adapter protein |
| UniProt Gene Name: | TIRAP |
| UniProt Entry Name: | TIRAP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TIRAP antibody (CAB12606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using TIRAP antibody (CAB12606) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using TIRAP antibody (CAB12606) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using TIRAP antibody (CAB12606) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using TIRAP antibody (CAB12606) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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