Cell Biology Antibodies 7

Anti-TIMP2 Antibody (CAB16439)

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SKU:
CAB16439
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TIMP2 Antibody
Antibody SKU:CAB16439
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TIMP2 (NP_003246.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse testis, Mouse brain, Rat testis
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TIMP2 (NP_003246.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VSLD VGGK KEYL IAGK AEGD GKMH ITLC DFIV PWDT LSTT QKKS LNHR YQMG CECK ITRC PMIP CYIS SPDE CLWM DWVT EKNI NGHQ AKFF ACIK RSDG S
Gene ID:7077
Uniprot:P16035
Cellular Location:Secreted
Calculated MW:24kDa
Observed MW:22KDa
Synonyms:TIMP2, CSC-21K, DDC8
Background:This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix.
UniProt Protein Function:TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis; Cell development/differentiation; Inhibitor

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: extracellular space; proteinaceous extracellular matrix; growth cone; cell surface; cell soma; extracellular region; basement membrane

Molecular Function:integrin binding; metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding; enzyme activator activity

Biological Process: response to drug; regulation of Rap protein signal transduction; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; central nervous system development; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging

NCBI Summary:This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]
UniProt Code:P16035
NCBI GenInfo Identifier:135854
NCBI Gene ID:7077
NCBI Accession:P16035.2
UniProt Secondary Accession:P16035,Q16121, Q93006, Q9UDF7,
UniProt Related Accession:P16035,Q96MC4
Molecular Weight:24,399 Da
NCBI Full Name:Metalloproteinase inhibitor 2
NCBI Synonym Full Names:TIMP metallopeptidase inhibitor 2
NCBI Official Symbol:TIMP2  
NCBI Official Synonym Symbols:DDC8; CSC-21K  
NCBI Protein Information:metalloproteinase inhibitor 2; TIMP-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2
UniProt Protein Name:Metalloproteinase inhibitor 2
UniProt Synonym Protein Names:CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2
Protein Family:Metalloproteinase inhibitor
UniProt Gene Name:TIMP2  
UniProt Entry Name:TIMP2_HUMAN
Anti-TIMP2 Antibody (CAB16439)Western blot analysis of extracts of various cell lines, using TIMP2 antibody (CAB16439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-TIMP2 Antibody (CAB16439)Immunohistochemistry of paraffin-embedded Rat ovary using TIMP2 antibody (CAB16439) at dilution of 1:100 (40x lens).
Anti-TIMP2 Antibody (CAB16439)Immunohistochemistry of paraffin-embedded Human breast cancer using TIMP2 antibody (CAB16439) at dilution of 1:150 (40x lens).
Anti-TIMP2 Antibody (CAB16439)Immunohistochemistry of paraffin-embedded Mouse pancreatic islet using TIMP2 antibody (CAB16439) at dilution of 1:150 (40x lens).
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