Immunology Antibodies 1
Anti-TIMP1 Antibody (CAB1389)
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- SKU:
- CAB1389
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-TIMP1 Antibody |
| Antibody SKU: | CAB1389 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-207 of human TIMP1 (NP_003245.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, SKOV3, HT-29, mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-207 of human TIMP1 (NP_003245.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CTCV PPHP QTAF CNSD LVIR AKFV GTPE VNQT TLYQ RYEI KMTK MYKG FQAL GDAA DIRF VYTP AMES VCGY FHRS HNRS EEFL IAGK LQDG LLHI TTCS FVAP WNSL SLAQ RRGF TKTY TVGC EECT VFPC LSIP CKLQ SGTH CLWT DQLL QGSE KGFQ SRHL ACLP REPG LCTW QSLR SQIA |
| Gene ID: | 7076 |
| Uniprot: | P01033 |
| Cellular Location: | Secreted |
| Calculated MW: | 23kDa |
| Observed MW: | 28kDa |
| Synonyms: | TIMP1, CLGI, EPA, EPO, HCI, TIMP, TIMP-1 |
| Background: | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. |
| UniProt Protein Function: | TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: Xp11.3-p11.23 Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane Molecular Function:metalloendopeptidase inhibitor activity; protein binding; growth factor activity; protease binding; metal ion binding; cytokine activity Biological Process: response to peptide hormone stimulus; platelet activation; extracellular matrix organization and biogenesis; negative regulation of metalloenzyme activity; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; blood coagulation; aging; negative regulation of apoptosis |
| NCBI Summary: | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P01033 |
| NCBI GenInfo Identifier: | 135850 |
| NCBI Gene ID: | 7076 |
| NCBI Accession: | P01033.1 |
| UniProt Secondary Accession: | P01033,Q14252, Q9UCU1, |
| UniProt Related Accession: | P01033 |
| Molecular Weight: | 23,171 Da |
| NCBI Full Name: | Metalloproteinase inhibitor 1 |
| NCBI Synonym Full Names: | TIMP metallopeptidase inhibitor 1 |
| NCBI Official Symbol: | TIMP1 |
| NCBI Official Synonym Symbols: | EPA; EPO; HCI; CLGI; TIMP |
| NCBI Protein Information: | metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1 |
| UniProt Protein Name: | Metalloproteinase inhibitor 1 |
| UniProt Synonym Protein Names: | Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1 |
| Protein Family: | Metalloproteinase inhibitor |
| UniProt Gene Name: | TIMP1 |
| UniProt Entry Name: | TIMP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TIMP1 antibody (CAB1389) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of extracts of mouse lung, using TIMP1 antibody (CAB1389) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using TIMP1 antibody (CAB1389) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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