Anti-Timm22 Antibody (CAB20813)
- SKU:
- CAB20813
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Goat
- Isotype:
- IgG
Description
| Antibody Name: | Anti-Timm22 Antibody (CAB20813) |
| Antibody SKU: | CAB20813 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunoprecipitation |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-121 of mouse Timm22 (NP_062792.2). |
| Application: | Western blotting, Immunoprecipitation |
| Recommended Dilution: | WB 1:500 - 1:2000 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | T47D, Mouse brain, Rat testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-121 of mouse Timm22 (NP_062792.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AEAP LQYS LLLQ YLVG DKRQ PRLL EPGS LGGI PSPA KSEE QKMI ERAM ESCA FKAV LACV GGFV LGGA FGIF TAGI DTNV GFDP KDPY RTPT AKEV LKDM GQR |
| Cellular Location: | |
| Calculated MW: | 20kDa |
| Observed MW: | 20KDa |
| Synonyms: | Tim22 |
| Background: | Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps (By similarity. |
 | Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug Timm22 antibody . Western blot was performed from the immunoprecipitate using Timm22 antibody at a dilition of 1:500. |
 | Western blot analysis of extracts of various cell lines, using Timm22 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
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