Cell Biology Antibodies 7
Anti-THPO Antibody (CAB1825)
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- SKU:
- CAB1825
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-THPO Antibody |
| Antibody SKU: | CAB1825 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-349 of human THPO (NP_001171068.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF7, U-937, BT-474 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-349 of human THPO (NP_001171068.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SPAP PACD LRVL SKLL RDSH VLHS RLSQ CPEV HPLP TPVL LPAV DFSL GEWK TQME ETKA QDIL GAVT LLLE GVMA ARGQ LGPT CLSS LLGQ LSGQ VRLL LGAL QSLL GTQG RTTA HKDP NAIF LSFQ HLLR GKVR FLML VGGS TLCV RRAP PTTA VPSR TSLV LTLN ELPN RTSG LLET NFTA SART TGSG LLKW QQGF RAKI PGLL NQTS RSLD QIPG YLNR IHEL LNGT RGLF PGPS RRTL GAPD ISSG TSDT GSLP PNLQ PGYS PSPT HPPT GQYT LFPL PPTL PTPV VQLH PLLP DPSA PTPT PTSP LLNT SYTH SQNL SQEG |
| Gene ID: | 7066 |
| Uniprot: | P40225 |
| Cellular Location: | Secreted |
| Calculated MW: | 33kDa/37kDa |
| Observed MW: | 32kDa |
| Synonyms: | THPO, MGDF, MKCSF, ML, MPLLG, THCYT1, TPO |
| Background: | Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. |
| UniProt Protein Function: | THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Secreted; Cytokine; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3q27 Cellular Component: extracellular space Molecular Function:growth factor activity; hormone activity; cytokine activity Biological Process: positive regulation of protein kinase B signaling cascade; cell proliferation; platelet activation; multicellular organismal development; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation Disease: Thrombocythemia 1 |
| NCBI Summary: | Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014] |
| UniProt Code: | P40225 |
| NCBI GenInfo Identifier: | 730982 |
| NCBI Gene ID: | 7066 |
| NCBI Accession: | P40225.1 |
| UniProt Related Accession: | P40225 |
| Molecular Weight: | |
| NCBI Full Name: | Thrombopoietin |
| NCBI Synonym Full Names: | thrombopoietin |
| NCBI Official Symbol: | THPO |
| NCBI Official Synonym Symbols: | ML; TPO; MGDF; MKCSF; MPLLG; THCYT1 |
| NCBI Protein Information: | thrombopoietin |
| UniProt Protein Name: | Thrombopoietin |
| UniProt Synonym Protein Names: | C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand |
| Protein Family: | Thyroid peroxidase |
| UniProt Gene Name: | THPO |
| UniProt Entry Name: | TPO_HUMAN |
 | Western blot analysis of extracts of various cell lines, using THPO antibody (CAB1825) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). |
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