Cell Death Antibodies 2

Nuclear Matrix Protein p84 (THOC1) Rabbit Polyclonal Antibody (CAB8179)

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SKU:
CAB8179
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
P84, HPR1, P84N5, DFNA86, Nuclear Matrix Protein p84 (THOC1)
Research Use:
Cell Death
Frequently bought together:

Description

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Nuclear Matrix Protein p84 (THOC1) Antibody (CAB8179)

The Nuclear Matrix Protein p84 (THOC1) Antibody (CAB8179) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the THOC1 protein, this antibody enables researchers to detect and analyze THOC1 expression in various cell types, making it an ideal choice for studies in molecular biology and gene expression regulation.THOC1 plays a crucial role in the transportation of mRNA from the nucleus to the cytoplasm, ensuring the proper functioning of gene expression processes.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Nuclear Matrix Protein p84 (THOC1) Antibody
SKU:CAB8179
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:TKKI LMGN EELT RLWN LCPD NMEA CKSE TREH MPTL EEFF EEAI EQAD PENM VENE YKAV NNSN YGWR ALRL LARR SPHF FQPT NQQF KSLP EYLE NMVI KLAK ELPP PSEE IKTG EDED EEDN DALL KENE SPDV RRDK PVTG EQIE VFAN KLGE QWKI LAPY LEMK DSEI RQIE CDSE DMKM RAKQ LLVA WQDQ EGVH ATPE NLIN ALNK SGLS DLAE SLTN DNET NS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:P84, HPR1, P84N5, DFNA86, Nuclear Matrix Protein p84 (THOC1)
Positive Sample:293T, Raji, Mouse spleen, Rat testis
Cellular Localization:Cytoplasm, Cytoplasm, Nucleus, Nucleus Matrix, Nucleus Speckle, Nucleoplasm.
Calculated MW:76kDa
Observed MW:85kDa

Predicted to enable DNA binding activity and RNA binding activity. Involved in several processes, including negative regulation of DNA damage checkpoint; regulation of nucleobase-containing compound metabolic process; and viral mRNA export from host cell nucleus. Located in cytoplasm and nuclear speck. Part of THO complex part of transcription export complex. Colocalizes with chromosome, telomeric region.

Purification MethodAffinity purification
Gene ID9984
RRIDAB_2772593
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Nuclear Matrix Protein p84 (THOC1) Rabbit pAb (CAB8179) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of various lysates using Nuclear Matrix Protein p84 (THOC1) Rabbit pAb (CAB8179) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse lung using Nuclear Matrix Protein p84 (THOC1) Rabbit pAb (CAB8179) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat ovary using Nuclear Matrix Protein p84 (THOC1) Rabbit pAb (CAB8179) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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