KO Validated Antibodies 1

Anti-TFRC Antibody (CAB18083)[KO Validated]

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SKU:
CAB18083
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TFRC Antibody [KO Validated]
Antibody SKU:CAB18083
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human TFRC (NP_001121620.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human TFRC (NP_001121620.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MMDQ ARSA FSNL FGGE PLSY TRFS LARQ VDGD NSHV EMKL AVDE EENA DNNT KANV TKPK RCSG SICY GTIA VIVF FLIG FMIG YLGY CKGV EPKT ECER LAGT ESPV REEP GEDF PAAR RLYW DDLK RKLS EKLD STDF
Gene ID:7037
Uniprot:P02786
Cellular Location:Cell membrane, Melanosome, Secreted, Single-pass type II membrane protein
Calculated MW:84kDa
Observed MW:105kDa
Anti-TFRC Antibody [KO Validated] (CAB18083)Western blot analysis of extracts from normal (control) and TFRC knockout (KO) HeLa cells, using TFRC antibody (CAB18083) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:TFRC, CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90
Background:This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.
UniProt Protein Function:TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system.
UniProt Protein Details:

Protein type:Receptor, misc.; Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: basolateral plasma membrane; cell surface; coated pit; cytoplasmic membrane-bound vesicle; endosome; external side of plasma membrane; extracellular region; extracellular space; integral to plasma membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; plasma membrane; recycling endosome

Molecular Function:double-stranded RNA binding; glycoprotein binding; identical protein binding; protein binding; protein homodimerization activity; transferrin receptor activity

Biological Process: regulation of cell growth; regulation of cell proliferation; transferrin transport

NCBI Summary:This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
UniProt Code:P02786
NCBI GenInfo Identifier:108935939
NCBI Gene ID:7037
NCBI Accession:P02786.2
UniProt Secondary Accession:P02786,Q1HE24, Q59G55, Q9UCN0, Q9UCU5, Q9UDF9, Q9UK21 D3DXB0,
UniProt Related Accession:P02786
Molecular Weight:84,871 Da
NCBI Full Name:Transferrin receptor protein 1
NCBI Synonym Full Names:transferrin receptor
NCBI Official Symbol:TFRC  
NCBI Official Synonym Symbols:T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46  
NCBI Protein Information:transferrin receptor protein 1
UniProt Protein Name:Transferrin receptor protein 1
UniProt Synonym Protein Names:T9; p90; CD_antigen: CD71
Protein Family:Transferrin receptor protein
UniProt Gene Name:TFRC  
UniProt Entry Name:TFR1_HUMAN
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