Cell Cycle Antibodies 1

Anti-TERF2 Antibody (CAB16316)

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SKU:
CAB16316
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-TERF2 Antibody
Antibody SKU:CAB16316
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human TERF2
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, HeLa, Mouse lung
Immunogen:Recombinant protein of human TERF2
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:7014
Uniprot:Q15554
Cellular Location:Chromosome, Nucleus, telomere
Calculated MW:32kDa/59kDa
Observed MW:70kDa
Synonyms:TERF2, TRBF2, TRF2
Background:This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic.
UniProt Protein Function:TRF2: Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single- stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology- directed repair (HDR), which can affect telomere length. Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct. Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; DNA repair, damage

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; male germ cell nucleus; Golgi apparatus; chromosome, telomeric region; nuclear telomere cap complex; Mre11 complex; nuclear chromosome, telomeric region; cytoplasm; nucleus

Molecular Function:protein C-terminus binding; protein binding; protein homodimerization activity; telomeric DNA binding; double-stranded telomeric DNA binding; chromatin binding

Biological Process: negative regulation of telomere maintenance; telomere capping; negative regulation of telomere maintenance via semi-conservative replication; in utero embryonic development; telomeric loop formation; positive regulation of nitric-oxide synthase activity; positive regulation of telomere maintenance; telomere maintenance via telomerase; cell cycle; age-dependent telomere shortening; telomere maintenance; protection from non-homologous end joining at telomere

NCBI Summary:This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq, Jul 2008]
UniProt Code:Q15554
NCBI GenInfo Identifier:613504836
NCBI Gene ID:7014
NCBI Accession:Q15554.3
UniProt Related Accession:Q15554
Molecular Weight:500
NCBI Full Name:Telomeric repeat-binding factor 2
NCBI Synonym Full Names:telomeric repeat binding factor 2
NCBI Official Symbol:TERF2  
NCBI Official Synonym Symbols:TRF2; TRBF2  
NCBI Protein Information:telomeric repeat-binding factor 2; telomeric DNA-binding protein; TTAGGG repeat-binding factor 2; telomeric repeat binding protein 2
UniProt Protein Name:Telomeric repeat-binding factor 2
UniProt Synonym Protein Names:TTAGGG repeat-binding factor 2; Telomeric DNA-binding protein
Protein Family:Tricorn protease-interacting factor
UniProt Gene Name:TERF2  
UniProt Entry Name:TERF2_HUMAN
Anti-TERF2 Antibody (CAB16316)Western blot analysis of extracts of various cell lines, using TERF2 antibody (CAB16316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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