Description
Antibody Name: | Anti-TEMT Antibody (CAB20962) |
Antibody SKU: | CAB20962 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Mouse |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Human TEMT. |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Mouse |
Positive Samples: | Mouse lung, Mouse liver, Mouse kidney |
Immunogen: | Recombinant protein of Human TEMT. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cytoplasm |
Calculated MW: | 28kDa |
Observed MW: | 29kDa |
Synonyms: | INMT, TEMT |
Background: | N-methylation of endogenous and xenobiotic compounds is a major method by which they are degraded. This gene encodes an enzyme that N-methylates indoles such as tryptamine. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream FAM188B (family with sequence similarity 188, member B) gene. |
Western blot analysis of extracts of various cell lines, using TEMT antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |