Epigenetics & Nuclear Signaling Antibodies 1

Anti-TDP2 Antibody (CAB11617)

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SKU:
CAB11617
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-TDP2 Antibody
Antibody SKU:CAB11617
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TDP2 (NP_057698.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HepG2, HeLa, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TDP2 (NP_057698.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MELG SCLE GGRE AAEE EGEP EVKK RRLL CVEF ASVA SCDA AVAQ CFLA ENDW EMER ALNS YFEP PVEE SALE RRPE TISE PKTY VDLT NEET TDST TSKI SPSE DTQQ ENGS MFSL ITWN IDGL DLNN LSER ARGV CSYL ALYS PDVI FLQE VIPP YYSY LKKR SSNY EIIT GHEE GYFT AIML KKSR VKLK SQEI IPFP
Gene ID:51567
Uniprot:O95551
Cellular Location:Cytoplasm, Nucleus, PML body, nucleolus
Calculated MW:32kDa/40kDa/44kDa
Observed MW:41kDa
Synonyms:TDP2, AD022, EAP2, EAPII, TTRAP, dJ30M3.3, hTDP2
Background:This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors.
UniProt Protein Function:TTRAP: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Has also 3'- tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'- tyrosyl-DNA phosphodiesterase in cells. Also acts as a 5'-tyrosyl- RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA. Also acts as an adapter by participating to the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF- beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non- canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation. Acts as a regulator of ribosome biogenesis following stress. Belongs to the CCR4/nocturin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nucleolus; Inhibitor; EC 3.1.4.-

Chromosomal Location of Human Ortholog: 6p22.3-p22.1

Cellular Component: nucleoplasm; PML body; intercellular bridge; cytoplasm; nucleolus; nucleus

Molecular Function:protein binding; nuclease activity; manganese ion binding; magnesium ion binding; transcription corepressor activity; single-stranded DNA binding

Biological Process: cell surface receptor linked signal transduction; double-strand break repair

NCBI Summary:This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. [provided by RefSeq, Jul 2008]
UniProt Code:O95551
NCBI GenInfo Identifier:67462008
NCBI Gene ID:51567
NCBI Accession:O95551.1
UniProt Secondary Accession:O95551,Q2TBE2, Q5JYM0, Q7Z6U5, Q9NUK5, Q9NYY9, B4DKL8 B4DQ95,
UniProt Related Accession:O95551
Molecular Weight:362
NCBI Full Name:Tyrosyl-DNA phosphodiesterase 2
NCBI Synonym Full Names:tyrosyl-DNA phosphodiesterase 2
NCBI Official Symbol:TDP2  
NCBI Official Synonym Symbols:EAP2; AD022; EAPII; TTRAP; hTDP2; dJ30M3.3  
NCBI Protein Information:tyrosyl-DNA phosphodiesterase 2; VPg unlinkase; ETS1-associated protein 2; ETS1-associated protein II; tyr-DNA phosphodiesterase 2; 5'-Tyr-DNA phosphodiesterase; tyrosyl-RNA phosphodiesterase; 5'-tyrosyl-DNA phosphodiesterase; TRAF and TNF receptor associ
UniProt Protein Name:Tyrosyl-DNA phosphodiesterase 2
UniProt Synonym Protein Names:5'-tyrosyl-DNA phosphodiesterase; 5'-Tyr-DNA phosphodiesterase; ETS1-associated protein 2; ETS1-associated protein II; EAPII; TRAF and TNF receptor-associated protein; Tyrosyl-RNA phosphodiesterase; VPg unlinkase
Protein Family:Tyrosyl-DNA phosphodiesterase
UniProt Gene Name:TDP2  
UniProt Entry Name:TYDP2_HUMAN
Anti-TDP2 Antibody (CAB11617)Western blot analysis of extracts of various cell lines, using TDP2 Antibody (CAB11617) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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