Antibody Name:	Anti-TCN2 Antibody
Antibody SKU:	CAB5755
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-250 of human TCN2 (NP_001171655.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	SW480, MCF7, A-549, U-251MG, Mouse kidney, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-250 of human TCN2 (NP_001171655.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EMCE IPEM DSHL VEKL GQHL LPWM DRLS LEHL NPSI YVGL RLSS LQAG TKED LYLH SLKL GYQQ CLLG SAFS EDDG DCQG KPSM GQLA LYLL ALRA NWHD HKGH PHTS YYQY GLGI LALC LHQK RVHD SVVD KLLY AVEP FHQG HHSV DTAA MAGL AFTC LKRS NFNP GRRQ RITM AIRT VREE ILKA QTPE GHFG NVYS TPLA LQFL MTSP MRGA ELGT ACLK ARVA LLAS
Gene ID:	6948
Uniprot:	P20062
Cellular Location:	Secreted
Calculated MW:	44kDa/47kDa
Observed MW:	54kDa

Synonyms:

TCN2, D22S676, D22S750, II, TC, TC II, TC-2, TC2, TCII

Background:

This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	TCN2: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells. Defects in TCN2 are the cause of transcobalamin II deficiency (TCN2 deficiency). This results in various forms of anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: lysosomal lumen; extracellular space; extracellular region; endosome Molecular Function:metal ion binding; cobalamin binding Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process Disease: Transcobalamin Ii Deficiency
NCBI Summary:	This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Code:	P20062
NCBI GenInfo Identifier:	224471876
NCBI Gene ID:	6948
NCBI Accession:	P20062.3
UniProt Secondary Accession:	P20062,Q96FD4, Q9BVI8, Q9UCI5, Q9UCI6, Q9UDM0,
UniProt Related Accession:	P20062,AAB25526
Molecular Weight:	Calculated MW: 44kDa/47kDaObserved MW: 54kDa
NCBI Full Name:	Transcobalamin-2
NCBI Synonym Full Names:	transcobalamin II
NCBI Official Symbol:	TCN2
NCBI Official Synonym Symbols:	II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
NCBI Protein Information:	transcobalamin-2; macrocytic anemia; vitamin B12-binding protein 2; transcobalamin II; macrocytic anemia
UniProt Protein Name:	Transcobalamin-2
UniProt Synonym Protein Names:	Transcobalamin II; TC II; TCII
Protein Family:	Transcobalamin
UniProt Gene Name:	TCN2
UniProt Entry Name:	TCO2_HUMAN

	Western blot analysis of extracts of various cell lines, using TCN2 antibody (CAB5755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using TCN2 antibody (CAB5755). Blue: DAPI for nuclear staining.