Developmental Biology

Anti-TCF4 Antibody (CAB1141)

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SKU:
CAB1141
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-TCF4 Antibody
Antibody SKU:CAB1141
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of human TCF4 (NP_001077431.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A375, K-562
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of human TCF4 (NP_001077431.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LRNH AVGP STAM PGGH GDMH GIIG PSHN GAMG GLGS GYGT GLLS ANRH SLMV GTHR EDGV ALRG SHSL LPNQ VPVP QLPV QSAT SPDL NPPQ DPYR GMPP G
Gene ID:6925
Uniprot:P15884
Cellular Location:Nucleus
Calculated MW:48-71kDa
Observed MW:60kDa
Synonyms:TCF4, E2-2, FECD3, ITF-2, ITF2, PTHS, SEF-2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, SEF2-1D, TCF-4, bHLHb19, TCF4
Background:This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described.
UniProt Protein Function:TCF4: Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'. Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2. Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: transcription factor complex; nucleus

Molecular Function:protein C-terminus binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription initiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; protein-DNA complex assembly

Disease: Corneal Dystrophy, Fuchs Endothelial, 3; Pitt-hopkins Syndrome

NCBI Summary:This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Aug 2011]
UniProt Code:P15884
NCBI GenInfo Identifier:3915747
NCBI Gene ID:6925
NCBI Accession:P15884.3
UniProt Secondary Accession:P15884,B3KT62, B3KUC0, B4DT37, B4DUG3, B7Z5M6, B7Z6Y1 G0LNT9, G0LNU0, G0LNU1, G0LNU2, G0LNU4,
UniProt Related Accession:P15884
Molecular Weight:48,098 Da
NCBI Full Name:Transcription factor 4
NCBI Synonym Full Names:transcription factor 4
NCBI Official Symbol:TCF4  
NCBI Official Synonym Symbols:E2-2; ITF2; PTHS; SEF2; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19  
NCBI Protein Information:transcription factor 4; SL3-3 enhancer factor 2; transcription factor 4, isoform C; transcription factor 4, isoform D; transcription factor 4, isoform E; transcription factor 4, isoform R; immunoglobulin transcription factor 2; class B basic helix-loop-helix protein 19
UniProt Protein Name:Transcription factor 4
UniProt Synonym Protein Names:Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2
Protein Family:Transcription factor
UniProt Gene Name:TCF4  
UniProt Entry Name:ITF2_HUMAN
Anti-TCF4 Antibody (CAB1141)Western blot analysis of extracts of various cell lines, using TCF4 antibody (CAB1141) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-TCF4 Antibody (CAB1141)Immunofluorescence analysis of C6 cells using TCF4 Rabbit pAb (CAB1141) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Anti-TCF4 Antibody (CAB1141)Immunofluorescence analysis of NIH-3T3 cells using TCF4 Rabbit pAb (CAB1141) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Anti-TCF4 Antibody (CAB1141)Immunofluorescence analysis of U-2 OS cells using TCF4 Rabbit pAb (CAB1141) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
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