Developmental Biology

Anti-TBCB Antibody (CAB13248)

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SKU:
CAB13248
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-TBCB Antibody
Antibody SKU:CAB13248
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human TBCB (NP_001272.2).
Application:WB IHC IF
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, 293T, Mouse brain, Mouse heart, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human TBCB (NP_001272.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEVT GVSA PTVT VFIS SSLN TFRS EKRY SRSL TIAE FKCK LELL VGSP ASCM ELEL YGVD DKFY SKLD QEDA LLGS YPVD DGCR IHVI DHSG ARLG EYED VSRV EKYT ISQE AYDQ RQDT VRSF LKRS KLGR YNEE ERAQ QEAE AAQR LAEE KAQA SSIP VGSR CEVR AAGQ SPRR GTVM YVGL TDFK PGYW IGVR YDEP LGKN DGSV NGKR YFEC QAKY GAFV KPAV VTVG DFPE EDYG LDEI
Gene ID:1155
Uniprot:Q99426
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:21kDa/27kDa
Observed MW:37kDa
Synonyms:TBCB, CG22, CKAP1, CKAPI
Background:
UniProt Protein Function:TBCB: a cytoskeletal protein that binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Chaperone; Cytoskeletal

Chromosomal Location of Human Ortholog: 19q13.11-q13.12

Cellular Component: microtubule cytoskeleton; nucleoplasm; microtubule; cytoplasm

Molecular Function:protein binding

Biological Process: nervous system development; 'de novo' posttranslational protein folding; cellular protein metabolic process; cell differentiation

UniProt Code:Q99426
NCBI GenInfo Identifier:3023518
NCBI Gene ID:1155
NCBI Accession:Q99426.2
UniProt Secondary Accession:Q99426,O00111, O00674, O14728, Q6FGY5,
UniProt Related Accession:Q99426
Molecular Weight:244
NCBI Full Name:Tubulin-folding cofactor B
NCBI Synonym Full Names:tubulin folding cofactor B
NCBI Official Symbol:TBCB  
NCBI Official Synonym Symbols:CG22; CKAP1; CKAPI  
NCBI Protein Information:tubulin-folding cofactor B; tubulin-specific chaperone B; cytoskeleton associated protein 1; cytoskeleton-associated protein CKAPI
UniProt Protein Name:Tubulin-folding cofactor B
UniProt Synonym Protein Names:Cytoskeleton-associated protein 1; Cytoskeleton-associated protein CKAPI; Tubulin-specific chaperone B
UniProt Gene Name:TBCB  
UniProt Entry Name:TBCB_HUMAN
Anti-TBCB Antibody (CAB13248)Western blot analysis of extracts of various cell lines, using TBCB antibody (CAB13248) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-TBCB Antibody (CAB13248)Immunohistochemistry of paraffin-embedded rat brain using TBCB antibody (CAB13248) at dilution of 1:100 (40x lens).
Anti-TBCB Antibody (CAB13248)Immunohistochemistry of paraffin-embedded human placenta using TBCB antibody (CAB13248) at dilution of 1:100 (40x lens).
Anti-TBCB Antibody (CAB13248)Immunohistochemistry of paraffin-embedded mouse kidney using TBCB antibody (CAB13248) at dilution of 1:100 (40x lens).
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