KO Validated Antibodies 1

Anti-TAGLN2 Antibody (CAB6761)[KO Validated]

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SKU:
CAB6761
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TAGLN2 Antibody [KO Validated]
Antibody SKU:CAB6761
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human TAGLN2 (NP_003555.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:293T, HeLa, THP-1, MCF-7, SKOV3, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human TAGLN2 (NP_003555.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MANR GPAY GLSR EVQQ KIEK QYDA DLEQ ILIQ WITT QCRK DVGR PQPG RENF QNWL KDGT VLCE LINA LYPE GQAP VKKI QAST MAFK QMEQ ISQF LQAA ERYG INTT DIFQ TVDL WEGK NMAC VQRT LMNL GGLA VARD DGLF SGDP NWFP KKSK ENPR NFSD NQLQ EGKN VIGL QMGT NRGA SQAG MTGY GMPR QIL
Gene ID:8407
Uniprot:P37802
Cellular Location:
Calculated MW:22kDa/24kDa
Observed MW:18kDa
Synonyms:TAGLN2, HA1756
Background:The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:TAGLN2: a cytoskeletal protein of the calponin family. One of the earliest markers of differentiated smooth muscle. Expressed in the smooth, cardiac, and skeletal muscle cells during early embryogenesis. Becomes restricted to vascular and visceral smooth muscle cells in late fetal development and adulthood. Its expression is abolished by activated Ras. Ras-dependent and Ras-independent mechanisms can cause the down-regulation of transgelin in breast and colon cancer cells lines and tumor samples.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21-q25

Cellular Component: vesicle

Molecular Function:protein binding

Biological Process: epithelial cell differentiation

NCBI Summary:The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]
UniProt Code:P37802
NCBI GenInfo Identifier:586000
NCBI Gene ID:8407
NCBI Accession:P37802.3
UniProt Secondary Accession:P37802,Q5JRQ6, Q5JRQ7, Q6FGI1, Q9BUH5, Q9H4P0, E9KL39
UniProt Related Accession:P37802
Molecular Weight:24,454 Da
NCBI Full Name:Transgelin-2
NCBI Synonym Full Names:transgelin 2
NCBI Official Symbol:TAGLN2  
NCBI Official Synonym Symbols:HA1756  
NCBI Protein Information:transgelin-2
UniProt Protein Name:Transgelin-2
UniProt Synonym Protein Names:Epididymis tissue protein Li 7e; SM22-alpha homolog
Protein Family:Transgelin
UniProt Gene Name:TAGLN2  
UniProt Entry Name:TAGL2_HUMAN
Anti-TAGLN2 Antibody [KO Validated] (CAB6761)Western blot analysis of extracts of various cell lines, using TAGLN2 antibody (CAB6761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-TAGLN2 Antibody [KO Validated] (CAB6761)Western blot analysis of extracts from normal (control) and TAGLN2 knockout (KO) 293T cells, using TAGLN2 antibody (CAB6761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-TAGLN2 Antibody [KO Validated] (CAB6761)Immunofluorescence analysis of MCF7 cells using TAGLN2 antibody (CAB6761). Blue: DAPI for nuclear staining.
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