Developmental Biology
Anti-SYT4 Antibody (CAB7737)
- SKU:
- CAB7737
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-SYT4 Antibody |
| Antibody SKU: | CAB7737 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 38-190 of human SYT4 (NP_065834.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, NIH/3T3, Mouse brain, Mouse spinal cord, Mouse eye, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 38-190 of human SYT4 (NP_065834.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CQRK SSKS NKTP PYKF VHVL KGVD IYPE NLNS KKKF GADD KNEV KNKP AVPK NSLH LDLE KRDL NGNF PKTN LKPG SPSD LENA TPKL FLEG EKES VSPE SLKS STSL TSEE KQEK LGTL FFSL EYNF ERKA FVVN IKEA RGLP AMDE QSMT S |
| Gene ID: | 6860 |
| Uniprot: | Q9H2B2 |
| Cellular Location: | Cytoplasmic vesicle, Single-pass membrane protein, secretory vesicle, synaptic vesicle membrane |
| Calculated MW: | 46kDa/47kDa |
| Observed MW: | 48kDa |
| Synonyms: | SYT4, HsT1192 |
| Background: |
| UniProt Protein Function: | SYT4: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Belongs to the synaptotagmin family. |
| UniProt Protein Details: | Protein type:Calcium-binding; Membrane protein, integral; Vesicle Chromosomal Location of Human Ortholog: 18q12.3 Cellular Component: intracellular membrane-bound organelle; plasma membrane Molecular Function:calcium ion binding; calcium-dependent phospholipid binding; clathrin binding; protein binding; syntaxin binding Biological Process: calcium ion-dependent exocytosis of neurotransmitter; regulation of calcium ion-dependent exocytosis; vesicle fusion |
| UniProt Code: | Q9H2B2 |
| NCBI GenInfo Identifier: | 18202937 |
| NCBI Gene ID: | 6860 |
| NCBI Accession: | Q9H2B2.1 |
| UniProt Secondary Accession: | Q9H2B2,Q9P2K4, B4DEU3, |
| UniProt Related Accession: | Q9H2B2 |
| Molecular Weight: | 46,064 Da |
| NCBI Full Name: | Synaptotagmin-4 |
| NCBI Synonym Full Names: | synaptotagmin 4 |
| NCBI Official Symbol: | SYT4 |
| NCBI Official Synonym Symbols: | HsT1192 |
| NCBI Protein Information: | synaptotagmin-4 |
| UniProt Protein Name: | Synaptotagmin-4 |
| UniProt Synonym Protein Names: | Synaptotagmin IV; SytIV |
| Protein Family: | Synaptotagmin |
| UniProt Gene Name: | SYT4 |
| UniProt Entry Name: | SYT4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SYT4 antibody (CAB7737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human stomach using SYT4 antibody (CAB7737) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using SYT4 antibody (CAB7737) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using SYT4 antibody (CAB7737) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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