Antibody Name:	STX16 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8168
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human STX16 (NP_001128244.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human
Positive Samples:	SH-SY5Y, MCF7, SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human STX16 (NP_001128244.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATR RLTD AFLL LRNN SIQN RQLL AEQV SSHI TSSP LHSR SIAA LADD RMAL VSGI SLDP EAAI GVTK RPPP KWVD GVDE IQYD VGRI KQKM KELA SLHD KHLN RPTL DDSS EEEH AIEI TTQE ITQL FHRC QRAV QALP SRAR ACSE QEGR LLGN VVAS LAQA LQEL STSF RHAQ SGYL KRMK NREE RSQH FFDT SVPL MDDG DDNT LYHR GFTE DQLV LVEQ NTLM VEER EREI RQIV QSIS DLNE IFRD LGAM IVEQ GTVL DRID YNVE QSCI KTED GLKQ LHKA EQYQ KKNR KMLV
Gene ID:	8675
Uniprot:	O14662
Cellular Location:	Cytoplasm, Golgi apparatus membrane, Single-pass type IV membrane protein
Calculated MW:	13kDa/31kDa/34kDa/35kDa/36kDa/37kDa
Observed MW:	44kDa

Synonyms:

STX16, SYN16

Background:

This gene encodes a protein that is a member of the syntaxin or t-SNARE (target-SNAP receptor) family. These proteins are found on cell membranes and serve as the targets for V-SNARES (vesicle-SNAP receptors) permitting specific synaptic vesicle docking and fusion. A microdeletion in the region of chromosome 20 where this gene is located has been associated with pseudohypoparathyroidism type Ib. Multiple transcript variants have been found for this gene. Read-through transcription also exists between this gene and the neighboring downstream aminopeptidase-like 1 (NPEPL1) gene.

UniProt Protein Function:	STX16: SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. Genetic variations in STX16 may be a cause of pseudohypoparathyroidism type 1B (PHP1B). Pseudohypoparathyroidism refers to a heterogeneous group of disorders characterized by resistance to parathyroid hormone (PTH). PHP1B is characterized by PTH-resistant hypocalcemia and hyperphosphatemia. Patients affected with PHP1B lack developmental defects characteristic of Albright hereditary osteodystrophy, and typically show no other endocrine abnormalities besides resistance to PTH. In some cases microdeletions involving STX16 appear to cause loss of methylation at exon A/B of the GNAS gene, resulting in PHP1B. Belongs to the syntaxin family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Vesicle Chromosomal Location of Human Ortholog: 20q13.32 Cellular Component: cytoplasm; focal adhesion; Golgi apparatus; Golgi cisterna; Golgi membrane; integral to membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; SNARE complex; trans-Golgi network; trans-Golgi network membrane Molecular Function:protein binding; SNAP receptor activity; syntaxin binding Biological Process: intracellular protein transport; retrograde transport, endosome to Golgi; vesicle docking; vesicle fusion Disease: Pseudohypoparathyroidism, Type Ib
NCBI Summary:	This gene encodes a protein that is a member of the syntaxin or t-SNARE (target-SNAP receptor) family. These proteins are found on cell membranes and serve as the targets for V-SNARES (vesicle-SNAP receptors) permitting specific synaptic vesicle docking and fusion. A microdeletion in the region of chromosome 20 where this gene is located has been associated with pseudohypoparathyroidism type Ib. Multiple transcript variants have been found for this gene. Read-through transcription also exists between this gene and the neighboring downstream aminopeptidase-like 1 (NPEPL1) gene. [provided by RefSeq, Mar 2011]
UniProt Code:	O14662
NCBI GenInfo Identifier:	85700437
NCBI Gene ID:	8675
NCBI Accession:	O14662.3
UniProt Secondary Accession:	O14662,O14661, O14663, O60517, A6NK32, A6NN69, A8MPP0 B7ZBN1, B7ZBN2, B7ZBN3, E1P5M0, E1P607,
UniProt Related Accession:	O14662
Molecular Weight:	31,074 Da
NCBI Full Name:	Syntaxin-16
NCBI Synonym Full Names:	syntaxin 16
NCBI Official Symbol:	STX16
NCBI Official Synonym Symbols:	SYN16
NCBI Protein Information:	syntaxin-16
UniProt Protein Name:	Syntaxin-16
Protein Family:	Syntaxin
UniProt Gene Name:	STX16
UniProt Entry Name:	STX16_HUMAN

	Western blot analysis of extracts of various cell lines, using STX16 antibody (CAB8168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human stomach using STX16 antibody (CAB8168) at dilution of 1:100 (40x lens).