Cell Biology Antibodies 12
Anti-Syntaxin-12 Antibody (CAB8200)
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- SKU:
- CAB8200
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | STX12 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8200 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human STX12 (NP_803173.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse brain, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human STX12 (NP_803173.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSYG PLDM YRNP GPSG PQLR DFSS IIQT CSGN IQRI SQAT AQIK NLMS QLGT KQDS SKLQ ENLQ QLQH STNQ LAKE TNEL LKEL GSLP LPLS TSEQ RQQR LQKE RLMN DFSA ALNN FQAV QRRV SEKE KESI ARAR AGSR LSAE ERQR EEQL VSFD SHEE WNQM QSQE DEVA ITEQ DLEL IKER ETAI RQLE ADIL DVNQ IFKD LAMM IHDQ GDLI DSIE ANVE SSEV HVER ATEQ LQRA AYYQ KKSR KK |
| Gene ID: | 23673 |
| Uniprot: | Q86Y82 |
| Cellular Location: | Cytoplasmic side, Endomembrane system, Endosome membrane, Golgi apparatus membrane, Single-pass type IV membrane protein |
| Calculated MW: | 31kDa |
| Observed MW: | 31kDa |
| Synonyms: | STX12, STX13, STX14 |
| Background: |
| UniProt Protein Function: | STX12: SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. The SNARE complex containing STX6, STX12, VAMP4 and VTI1A mediates vesicle fusion (in vitro). Belongs to the syntaxin family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Vesicle Chromosomal Location of Human Ortholog: 1p35.3 Cellular Component: endomembrane system; integral to membrane; lipid raft; phagocytic vesicle; SNARE complex; vesicle Molecular Function:protein binding; SNAP receptor activity; SNARE binding Biological Process: autophagic vacuole formation; cholesterol efflux; intracellular protein transport; protein stabilization; vesicle docking; vesicle fusion |
| UniProt Code: | Q86Y82 |
| NCBI GenInfo Identifier: | 47117211 |
| NCBI Gene ID: | 23673 |
| NCBI Accession: | Q86Y82.1 |
| UniProt Secondary Accession: | Q86Y82,O95564, B1AJQ7, |
| UniProt Related Accession: | Q86Y82 |
| Molecular Weight: | 31,642 Da |
| NCBI Full Name: | Syntaxin-12 |
| NCBI Synonym Full Names: | syntaxin 12 |
| NCBI Official Symbol: | STX12 |
| NCBI Official Synonym Symbols: | STX13; STX14 |
| NCBI Protein Information: | syntaxin-12 |
| UniProt Protein Name: | Syntaxin-12 |
| Protein Family: | Syntaxin |
| UniProt Gene Name: | STX12 |
| UniProt Entry Name: | STX12_HUMAN |
 | Western blot analysis of extracts of various cell lines, using STX12 antibody (CAB8200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using STX12 antibody (CAB8200) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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