Description
Antibody Name: | Anti-Syndecan 3 Antibody (CAB20929) |
Antibody SKU: | CAB20929 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Human Syndecan 3. |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | U-251MG, MCF7, A-549, Mouse lung, Mouse spleen, Rat lung |
Immunogen: | Recombinant protein of Human Syndecan 3. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | |
Calculated MW: | 45kDa |
Observed MW: | 70KDa |
Synonyms: | SDC3, SDCN, SYND3 |
Background: | The protein encoded by this gene belongs to the syndecan proteoglycan family. It may play a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity. |
Western blot analysis of extracts of various cell lines, using Syndecan 3 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |