Epigenetics & Nuclear Signaling Antibodies 4

Anti-SUPT5H Antibody (CAB9193)

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SKU:
CAB9193
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SUPT5H Antibody
Antibody SKU:CAB9193
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 125-425 of human SUPT5H (NP_003160.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, HeLa, A-549, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 125-425 of human SUPT5H (NP_003160.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LQNL WRDQ REEE LGEY YMKK YAKS SVGE TVYG GSDE LSDD ITQQ QLLP GVKD PNLW TVKC KIGE ERAT AISL MRKF IAYQ FTDT PLQI KSVV APEH VKGY IYVE AYKQ THVK QAIE GVGN LRLG YWNQ QMVP IKEM TDVL KVVK EVAN LKPK SWVR LKRG IYKD DIAQ VDYV EPSQ NTIS LKMI PRID YDRI KARM SLKD WFAK RKKF KRPP QRLF DAEK IRSL GGDV ASDG DFLI FEGN RYSR KGFL FKSF AMSA VITE GVKP TLSE LEKF EDQP EGID LEVV TEST GKER EHNF QPGD N
Gene ID:6829
Uniprot:O00267
Cellular Location:Nucleus
Calculated MW:120kDa/121kDa
Observed MW:160kDa
Synonyms:SUPT5H, SPT5, SPT5H, Tat-CT1
Background:
UniProt Protein Function:SPT5: a transcription elongation factor. Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:RNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: nucleoplasm; nucleus

Molecular Function:enzyme binding; protein binding; protein heterodimerization activity

Biological Process: mRNA capping; negative regulation of RNA elongation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of macroautophagy; positive regulation of RNA elongation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of viral transcription; response to organic substance; RNA elongation; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter

UniProt Code:O00267
NCBI GenInfo Identifier:74735318
NCBI Gene ID:6829
NCBI Accession:O00267.1
UniProt Secondary Accession:O00267,O43279, Q59G52, Q99639,
UniProt Related Accession:O00267
Molecular Weight:120,499 Da
NCBI Full Name:Transcription elongation factor SPT5
NCBI Synonym Full Names:SPT5 homolog, DSIF elongation factor subunit
NCBI Official Symbol:SUPT5H  
NCBI Official Synonym Symbols:SPT5; SPT5H; Tat-CT1  
NCBI Protein Information:transcription elongation factor SPT5
UniProt Protein Name:Transcription elongation factor SPT5
UniProt Synonym Protein Names:DRB sensitivity-inducing factor 160 kDa subunit; DSIF p160; DRB sensitivity-inducing factor large subunit; DSIF large subunit; Tat-cotransactivator 1 protein; Tat-CT1 protein
UniProt Gene Name:SUPT5H  
UniProt Entry Name:SPT5H_HUMAN
Anti-SUPT5H Polyclonal Antibody (CAB9193)Western blot analysis of extracts of various cell lines, using SUPT5H antibody (CAB9193) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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