Antibody Name:	Anti-STUB1 Antibody
Antibody SKU:	CAB11751
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human STUB1 (NP_005852.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, OVCAR3, HT-1080, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human STUB1 (NP_005852.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKGK EEKE GGAR LGAG GGSP EKSP SAQE LKEQ GNRL FVGR KYPE AAAC YGRA ITRN PLVA VYYT NRAL CYLK MQQH EQAL ADCR RALE LDGQ SVKA HFFL GQCQ LEME SYDE AIAN LQRA YSLA KEQR LNFG DDIP SALR IAKK KRWN SIEE RRIH QESE LHSY LSRL IAAE RERE LEEC QRNH EGDE DDSH VRAQ QACI EAKH DKYM ADMD ELFS QVDE KRKK RDIP DYLC GKIS FELM REPC ITPS GITY DRKD IEEH LQRV GHFD PVTR SPLT QEQL IPNL AMKE VIDA FISE NGWV EDY
Gene ID:	10273
Uniprot:	Q9UNE7
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	27kDa/34kDa
Observed MW:	36kDa

Synonyms:

STUB1, CHIP, HSPABP2, NY-CO-7, SCAR16, SDCCAG7, UBOX1

Background:

This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2.

UniProt Protein Function:	CHIP: E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF- BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Homodimer. Interacts with BAG2, and with the E2 ubiquitin conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Interacts with the C-terminal domains of HSPA8 and HSPA1A. Detected in a ternary complex containing STUB1, HSPA1A and HSPBP1. Interacts with MKKS. Interacts with DYX1C1 and POLB. Interacts (via TPR repeats) with HSP90AA1. Interacts (when monoubiquitinated) with ATXN3. Interacts with UBE2W. Interacts (via the U-box domain) with the UBE2V2- UBE2N heterodimer; the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with DNAJB6. Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Adaptor/scaffold Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: nucleoplasm; intermediate filament cytoskeleton; endoplasmic reticulum; ubiquitin conjugating enzyme complex; cytoplasm; plasma membrane; cytosol; ubiquitin ligase complex; nuclear inclusion body Molecular Function:protein binding, bridging; protein homodimerization activity; ubiquitin-protein ligase activity; misfolded protein binding; Hsp90 protein binding; Hsp70 protein binding; protein binding; enzyme binding; G-protein-coupled receptor binding; TPR domain binding; ubiquitin protein ligase binding; SMAD binding; kinase binding; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein polyubiquitination; unfolded protein response; protein maturation; misfolded or incompletely synthesized protein catabolic process; DNA repair; ubiquitin-dependent SMAD protein catabolic process; positive regulation of protein ubiquitination; transforming growth factor beta receptor signaling pathway; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity; regulation of glucocorticoid metabolic process; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of protein binding Disease: Spinocerebellar Ataxia, Autosomal Recessive 16
NCBI Summary:	This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2. [provided by RefSeq, Jun 2014]
UniProt Code:	Q9UNE7
NCBI GenInfo Identifier:	78099173
NCBI Gene ID:	10273
NCBI Accession:	Q9UNE7.2
UniProt Related Accession:	Q9UNE7
Molecular Weight:
NCBI Full Name:	E3 ubiquitin-protein ligase CHIP
NCBI Synonym Full Names:	STIP1 homology and U-box containing protein 1
NCBI Official Symbol:	STUB1
NCBI Official Synonym Symbols:	CHIP; SCA48; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
NCBI Protein Information:	E3 ubiquitin-protein ligase CHIP
UniProt Protein Name:	E3 ubiquitin-protein ligase CHIP
UniProt Synonym Protein Names:	Antigen NY-CO-7; CLL-associated antigen KW-8; Carboxy terminus of Hsp70-interacting protein; STIP1 homology and U box-containing protein 1
Protein Family:	Chitoporin
UniProt Gene Name:	STUB1
UniProt Entry Name:	CHIP_HUMAN

	Western blot analysis of extracts of various cell lines, using STUB1 Antibody (CAB11751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using STUB1 Polyclonal Antibody (CAB11751) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using STUB1 Polyclonal Antibody (CAB11751) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using STUB1 Polyclonal Antibody (CAB11751) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.