Anti-STOP1 Antibody (CAB21044)

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SKU:
CAB21044
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-STOP1 Antibody (CAB21044)
Antibody SKU:CAB21044
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Arabidopsis thaliana
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 55-180 of arabidopsis thaliana STOP1 (NP_174697.1).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Arabidopsis thaliana
Positive Samples:Inflorescences
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 55-180 of arabidopsis thaliana STOP1 (NP_174697.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PGLQ ESIQ ANVD FLQG VRAQ AWDP RTML SNLS FMEQ KIHQ LQDL VHLL VGRG GQLQ GRQD ELAA QQQQ LITT DLTS IIIQ LIST AGSL LPSV KHNM STAP GPFT GQPG SAVF PYVR EANN VASQ SQ
Cellular Location:
Calculated MW:
Observed MW:55KDa
Synonyms:AtSTOP1, F7P12.7, F7P12_7, sensitive to proton rhizotoxicity 1
Background:Encodes a putative nuclear Cys(2)His(2)-type zinc finger protein involved in H+ and Al3+ rhizotoxicity. In mutants exposed to aluminum stress, there is no induction of AtALMT1, an malate transporter known to be involved in the mediation of aluminum toxicity. Cell wall of the mutant is unstable in low pH medium (pH 4.5) in low Ca solution. This would mediate Ca-alleviation of low pH stress through pectin-Ca interaction.
Western blot analysis of extracts of Inflorescences, using STOP1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Western blot analysis of extracts of Inflorescences, using STOP1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
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