Antibody Name:	STK38 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8191
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human STK38 (NP_009202.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	A-549, SW480, 293T, MCF7, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human STK38 (NP_009202.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAMT GSTP CSSM SNHT KERV TMTK VTLE NFYS NLIA QHEE REMR QKKL EKVM EEEG LKDE EKRL RRSA HARK ETEF LRLK RTRL GLED FESL KVIG RGAF GEVR LVQK KDTG HVYA MKIL RKAD MLEK EQVG HIRA ERDI LVEA DSLW VVKM FYSF QDKL NLYL IMEF LPGG DMMT LLMK KDTL TEEE TQFY IAET VLAI
Gene ID:	11329
Uniprot:	Q15208
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	54kDa
Observed MW:	60kDa

Synonyms:

STK38, NDR, NDR1

Background:

This gene encodes a member of the AGC serine/threonine kinase family of proteins. The kinase activity of this protein is regulated by autophosphorylation and phosphorylation by other upstream kinases. This protein has been shown to function in the cell cycle and apoptosis. This protein has also been found to regulate the protein stability and transcriptional activity of the MYC oncogene. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NDR1: is an AGC kinase of the NDR family. An apparent upstream regulator of Myc activity in human B-cell lymphoma. Mediates apoptosis downstream of the tumor suppressor proteins RASSF1A and MST1. Reduced expression of NDR1 results in defective apoptotic responses and a predisposition to develop T cell lymphoma in mice. Activated by binding of S100B which releases autoinhibitory N-lobe interactions. Ubiquitously expressed with highest levels observed in peripheral blood leukocytes.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.1; AGC group; NDR family Chromosomal Location of Human Ortholog: 6p21 Cellular Component: cytoplasm; nucleus Molecular Function:ATP binding; magnesium ion binding; mitogen-activated protein kinase kinase kinase binding; protein binding; protein serine/threonine kinase activity Biological Process: negative regulation of MAP kinase activity; peptidyl-serine phosphorylation; protein amino acid phosphorylation; protein modification process
NCBI Summary:	This gene encodes a member of the AGC serine/threonine kinase family of proteins. The kinase activity of this protein is regulated by autophosphorylation and phosphorylation by other upstream kinases. This protein has been shown to function in the cell cycle and apoptosis. This protein has also been found to regulate the protein stability and transcriptional activity of the MYC oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
UniProt Code:	Q15208
NCBI GenInfo Identifier:	56749457
NCBI Gene ID:	11329
NCBI Accession:	Q15208.1
UniProt Secondary Accession:	Q15208,Q503A1,
UniProt Related Accession:	Q15208
Molecular Weight:	54,190 Da
NCBI Full Name:	Serine/threonine-protein kinase 38
NCBI Synonym Full Names:	serine/threonine kinase 38
NCBI Official Symbol:	STK38
NCBI Official Synonym Symbols:	NDR; NDR1
NCBI Protein Information:	serine/threonine-protein kinase 38
UniProt Protein Name:	Serine/threonine-protein kinase 38
UniProt Synonym Protein Names:	NDR1 protein kinase; Nuclear Dbf2-related kinase 1
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	STK38
UniProt Entry Name:	STK38_HUMAN

	Western blot analysis of extracts of various cell lines, using STK38 antibody (CAB8191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using STK38 antibody (CAB8191) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using STK38 antibody (CAB8191) at dilution of 1:100. Blue: DAPI for nuclear staining.