Anti-Sterol carrier protein 2 Antibody (CAB9430)

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SKU:
CAB9430
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-Sterol carrier protein 2 Antibody (CAB9430)
Antibody SKU:CAB9430
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Sterol carrier protein 2.
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HepG2, U-87MG, Rat liver, Rat brain
Immunogen:A synthesized peptide derived from human Sterol carrier protein 2.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Cytoplasm, Mitochondrion, Mitochondrion, Peroxisome
Calculated MW:58kDa
Observed MW:59KDa
Synonyms:SCP2, NLTP, NSL-TP, SCP-2, SCP-CHI, SCP-X, SCPX, sterol carrier protein 2
Background:This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.
Immunofluorescence analysis of NIH/3T3 cells using Sterol carrier protein 2 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using Sterol carrier protein 2 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using Sterol carrier protein 2 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.Immunofluorescence analysis of PC-12 cells using Sterol carrier protein 2 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using Sterol carrier protein 2 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.Immunofluorescence analysis of HepG2 cells using Sterol carrier protein 2 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Sterol carrier protein 2 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.Immunofluorescence analysis of U2OS cells using Sterol carrier protein 2 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat brain using Sterol carrier protein 2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded rat brain using Sterol carrier protein 2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using Sterol carrier protein 2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded mouse kidney using Sterol carrier protein 2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung cancer using Sterol carrier protein 2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human lung cancer using Sterol carrier protein 2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using Sterol carrier protein 2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human liver cancer using Sterol carrier protein 2 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
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