Antibody Name:	Anti-SRI Antibody
Antibody SKU:	CAB6751
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human SRI (NP_003121.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, MCF7, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human SRI (NP_003121.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAYP GHPG AGGG YYPG GYGG APGG PAFP GQTQ DPLY GYFA AVAG QDGQ IDAD ELQR CLTQ SGIA GGYK PFNL ETCR LMVS MLDR DMSG TMGF NEFK ELWA VLNG WRQH FISF DTDR SGTV DPQE LQKA LTTM GFRL SPQA VNSI AKRY STNG KITF DDYI ACCV KLRA LTDS FRRR DTAQ QGVV NFPY DDFI QCVM SV
Gene ID:	6717
Uniprot:	P30626
Cellular Location:	Cytoplasm, Cytoplasmic side, Peripheral membrane protein, Sarcoplasmic reticulum membrane
Calculated MW:	20kDa/21kDa
Observed MW:	22kDa

Synonyms:

SRI, CP-22, CP22, SCN, V19, sorcin

Background:

This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene.

UniProt Protein Function:	SRI: Calcium-binding protein that modulates excitation- contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.
UniProt Protein Details:	Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 7q21.1 Cellular Component: nucleoplasm; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; smooth endoplasmic reticulum; membrane; mitochondrion; sarcoplasmic reticulum; T-tubule; cytoplasm; nerve terminal; cytosol; Z disc Molecular Function:protein binding; protease binding; protein heterodimerization activity; calcium channel regulator activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; receptor binding Biological Process: regulation of action potential; cytoplasmic sequestering of transcription factor; muscle development; negative regulation of heart rate; heart development; regulation of striated muscle contraction; regulation of calcium ion transport; proteolysis; signal transduction; regulation of heart contraction; transport; calcium ion transport; positive regulation of release of sequestered calcium ion into cytosol; intracellular sequestering of iron ion
NCBI Summary:	This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:	P30626
NCBI GenInfo Identifier:	267021
NCBI Gene ID:	6717
NCBI Accession:	P30626.1
UniProt Secondary Accession:	P30626,A8MTH6, B4DKK2, D6W5Q0,
UniProt Related Accession:	P30626
Molecular Weight:	198
NCBI Full Name:	Sorcin
NCBI Synonym Full Names:	sorcin
NCBI Official Symbol:	SRI
NCBI Official Synonym Symbols:	SCN; V19; CP22; CP-22
NCBI Protein Information:	sorcin; H_RG167B05.1; 22 kDa protein; calcium binding protein amplified in mutlidrug-resistant cells
UniProt Protein Name:	Sorcin
UniProt Synonym Protein Names:	22 kDa protein; CP-22; CP22; V19
UniProt Gene Name:	SRI
UniProt Entry Name:	SORCN_HUMAN

	Western blot analysis of extracts of various cell lines, using SRI Antibody (CAB6751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using SRI antibody (CAB6751) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using SRI antibody (CAB6751) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using SRI antibody (CAB6751) at dilution of 1:100 (40x lens).