Metabolism Antibodies 1

Anti-SREBP1c Antibody (CAB15586)

(No reviews yet) Write a Review
SKU:
CAB15586
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SREBP1c Antibody
Antibody SKU:CAB15586
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SREBF1 (NP_001005291.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Mouse kidney, Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SREBF1 (NP_001005291.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDEP PFSE AALE QALG EPCD LDAA LLTD IEGE VGAG RGRA NGLD APRA GADR GAMD CTFE DMLQ LINN QDSD FPGL FDPP YAGS GAGG TDPA SPDT SSPG
Gene ID:6720
Uniprot:P36956
Cellular Location:COPII-coated vesicle membrane, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein, Multi-pass membrane protein, Nucleus
Calculated MW:45kDa/48kDa/111kDa/113kDa/121kDa/124kDa
Observed MW:70kDa
Synonyms:SREBF1, SREBP-1c, SREBP1, SREBP1a, bHLHd1
Background:This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17.
UniProt Protein Function:Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
NCBI Summary:This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Mar 2016]
UniProt Code:P36956
NCBI GenInfo Identifier:166897633
NCBI Gene ID:6720
NCBI Accession:P36956.2
UniProt Secondary Accession:P36956,Q16062, Q59F52, Q6P4R7, Q6PFW7, Q6PJ36, Q8TAK9 B0I4X3, B0I4X4, D3DXC4,
UniProt Related Accession:P36956
Molecular Weight:45,816 Da
NCBI Full Name:Sterol regulatory element-binding protein 1
NCBI Synonym Full Names:sterol regulatory element binding transcription factor 1
NCBI Official Symbol:SREBF1  
NCBI Official Synonym Symbols:SREBP1; bHLHd1; SREBP1a; SREBP-1c  
NCBI Protein Information:sterol regulatory element-binding protein 1
UniProt Protein Name:Sterol regulatory element-binding protein 1
UniProt Synonym Protein Names:Class D basic helix-loop-helix protein 1; bHLHd1
UniProt Gene Name:SREBF1  
UniProt Entry Name:SRBP1_HUMAN
Anti-SREBP1c Antibody (CAB15586)Western blot analysis of extracts of various cell lines, using SREBP1c antibody (CAB15586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
View AllClose

0 Reviews

View AllClose

Related Products