Metabolism Antibodies 2
Anti-SQS/FDFT1 Antibody (CAB6229)
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- SKU:
- CAB6229
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-SQS/FDFT1 Antibody |
| Antibody SKU: | CAB6229 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SQS/FDFT1 (NP_004453.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1, HT-29, Jurkat, MCF7, HepG2, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SQS/FDFT1 (NP_004453.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEFV KCLG HPEE FYNL VRFR IGGK RKVM PKMD QDSL SSSL KTCY KYLN QTSR SFAA VIQA LDGE MRNA VCIF YLVL RALD TLED DMTI SVEK KVPL LHNF HSFL YQPD WRFM ESKE KDRQ VLED FPTI SLEF RNLA EKYQ TVIA DICR RMGI GMAE FLDK HVTS EQEW DKYC HYVA GLVG IGLS RLFS ASEF EDPL VGED TERA NSMG LFLQ KTNI IRDY LEDQ QGGR EFWP QEVW SRYV KKLG DFAK PENI DLAV QCLN |
| Gene ID: | 2222 |
| Uniprot: | P37268 |
| Cellular Location: | Endoplasmic reticulum membrane, Multi-pass membrane protein |
| Calculated MW: | 35kDa/38kDa/40kDa/43kDa/48kDa |
| Observed MW: | 48kDa |
| Synonyms: | FDFT1, DGPT, ERG9, SQS, SS |
| Background: | This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. |
| UniProt Protein Function: | FDFT1: a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 2.5.1.21; Endoplasmic reticulum; Lipid Metabolism - steroid biosynthesis; Membrane protein, integral; Transferase; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 8p23.1-p22 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane Molecular Function:farnesyl-diphosphate farnesyltransferase activity Biological Process: cholesterol biosynthetic process; steroid biosynthetic process |
| NCBI Summary: | This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P37268 |
| NCBI GenInfo Identifier: | 585126 |
| NCBI Gene ID: | 2222 |
| NCBI Accession: | P37268.1 |
| UniProt Secondary Accession: | P37268,Q96GT0, B3KQ95, B4DJE5, B4DT56, B7Z1J3, |
| UniProt Related Accession: | P37268 |
| Molecular Weight: | 43,060 Da |
| NCBI Full Name: | Squalene synthase |
| NCBI Synonym Full Names: | farnesyl-diphosphate farnesyltransferase 1 |
| NCBI Official Symbol: | FDFT1 |
| NCBI Official Synonym Symbols: | SS; SQS; DGPT; ERG9 |
| NCBI Protein Information: | squalene synthase |
| UniProt Protein Name: | Squalene synthase |
| UniProt Synonym Protein Names: | FPP:FPP farnesyltransferase; Farnesyl-diphosphate farnesyltransferase |
| UniProt Gene Name: | FDFT1 |
| UniProt Entry Name: | FDFT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SQS/FDFT1 antibody (CAB6229) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded mouse testis using SQS/FDFT1 antibody (CAB6229) at dilution of 1:100 (20x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using SQS/FDFT1 antibody (CAB6229) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using SQS/FDFT1 antibody (CAB6229) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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