Cell Biology Antibodies 2
Anti-SPRR3 Antibody (CAB12041)
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- SKU:
- CAB12041
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SPRR3 Antibody |
| Antibody SKU: | CAB12041 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human SPRR3 (NP_005407.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IF 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human SPRR3 (NP_005407.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSSY QQKQ TFTP PPQL QQQQ VKQP SQPP PQEI FVPT TKEP CHSK VPQP GNTK IPEP GCTK VPEP GCTK VP |
| Gene ID: | 6707 |
| Uniprot: | Q9UBC9 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 18kDa |
| Observed MW: | 26kDa |
| Synonyms: | SPRR3 |
| Background: |
| UniProt Protein Function: | SPRR3: Cross-linked envelope protein of keratinocytes. Belongs to the cornifin (SPRR) family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 1q21-q22 Cellular Component: cornified envelope; cytoplasm Molecular Function:protein binding; structural molecule activity Biological Process: keratinocyte differentiation; peptide cross-linking; wound healing |
| UniProt Code: | Q9UBC9 |
| NCBI GenInfo Identifier: | 20138065 |
| NCBI Gene ID: | 6707 |
| NCBI Accession: | Q9UBC9.2 |
| UniProt Secondary Accession: | Q9UBC9,O75597, Q4ZGI7, Q5T525, Q8NET7, Q9UDG3, A5YKK8 B2R4G8, D3DV32, |
| UniProt Related Accession: | Q9UBC9 |
| Molecular Weight: | 18,154 Da |
| NCBI Full Name: | Small proline-rich protein 3 |
| NCBI Synonym Full Names: | small proline rich protein 3 |
| NCBI Official Symbol: | SPRR3 |
| NCBI Protein Information: | small proline-rich protein 3 |
| UniProt Protein Name: | Small proline-rich protein 3 |
| UniProt Synonym Protein Names: | 22 kDa pancornulin; Cornifin beta; Esophagin |
| Protein Family: | Small proline-rich protein |
| UniProt Gene Name: | SPRR3 |
| UniProt Entry Name: | SPRR3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SPRR3 antibody (CAB12041) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using SPRR3 Rabbit pAb (CAB12041) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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