Antibody Name:	Anti-SPPL2A Antibody
Antibody SKU:	CAB15539
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 421-520 of human SPPL2A (NP_116191.2).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, Mouse brain, Rat small intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 421-520 of human SPPL2A (NP_116191.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AYCR RFDV QTGS SYIY YVSS TVAY AIGM ILTF VVLV LMKK GQPA LLYL VPCT LITA SVVA WRRK EMKK FWKG NSYQ MMDH LDCA TNEE NPVI SGEQ IVQQ
Gene ID:	84888
Uniprot:	Q8TCT8
Cellular Location:	Late endosome membrane, Lumenal side, Lysosome membrane, Membrane, Multi-pass membrane protein
Calculated MW:	58kDa
Observed MW:	57kDa

Synonyms:

SPPL2A, IMP3, PSL2

Background:

This gene encodes a member of the GXGD family of aspartic proteases, which are transmembrane proteins with two conserved catalytic motifs localized within the membrane-spanning regions, as well as a member of the signal peptide peptidase-like protease (SPPL) family. This protein is expressed in all major adult human tissues and localizes to late endosomal compartments and lysosomal membranes. A pseudogene of this gene also lies on chromosome 15.

UniProt Protein Function:	SPPL2A: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). May play a role in the regulation of innate and adaptive immunity. Belongs to the peptidase A22B family.
UniProt Protein Details:	Protein type:Cancer Testis Antigen (CTA); EC 3.4.23.-; Membrane protein, multi-pass; Protease; Membrane protein, integral Chromosomal Location of Human Ortholog: 15q21.2 Cellular Component: intracellular membrane-bound organelle; late endosome; lysosomal membrane; membrane; plasma membrane Molecular Function:aspartic endopeptidase activity, intramembrane cleaving; protein binding; protein homodimerization activity Biological Process: membrane protein ectodomain proteolysis; membrane protein intracellular domain proteolysis; membrane protein proteolysis; regulation of immune response; signal peptide processing
NCBI Summary:	This gene encodes a member of the GXGD family of aspartic proteases, which are transmembrane proteins with two conserved catalytic motifs localized within the membrane-spanning regions, as well as a member of the signal peptide peptidase-like protease (SPPL) family. This protein is expressed in all major adult human tissues and localizes to late endosomal compartments and lysosomal membranes. A pseudogene of this gene also lies on chromosome 15. [provided by RefSeq, Feb 2012]
UniProt Code:	Q8TCT8
NCBI GenInfo Identifier:	25008981
NCBI Gene ID:	84888
NCBI Accession:	Q8TCT8.2
UniProt Secondary Accession:	Q8TCT8,Q8TAW1, Q96SZ8, B2RDS0,
UniProt Related Accession:	Q8TCT8
Molecular Weight:	58,143 Da
NCBI Full Name:	Signal peptide peptidase-like 2A
NCBI Synonym Full Names:	signal peptide peptidase like 2A
NCBI Official Symbol:	SPPL2A
NCBI Official Synonym Symbols:	IMP3; PSL2
NCBI Protein Information:	signal peptide peptidase-like 2A
UniProt Protein Name:	Signal peptide peptidase-like 2A
UniProt Synonym Protein Names:	Intramembrane protease 3
Protein Family:	Signal peptide peptidase
UniProt Gene Name:	SPPL2A
UniProt Entry Name:	SPP2A_HUMAN

	Western blot analysis of extracts of various cell lines, using SPPL2A antibody (CAB15539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded Human colon using SPPL2A Rabbit pAb (CAB15539) at dilution of 1:100 (40x lens).