Antibody Name:	Anti-SPN Antibody
Antibody SKU:	CAB6412
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 276-400 of human SPN (NP_003114.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 276-400 of human SPN (NP_003114.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LWRR RQKR RTGA LVLS RGGK RNGV VDAW AGPA QVPE EGAV TVTV GGSG GDKG SGFP DGEG SSRR PTLT TFFG RRKS RQGS LAME ELKS GSGP SLKG EEEP LVAS EDGA VDAP APDE PEGG DGAA P
Gene ID:	6693
Uniprot:	P16150
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	40kDa
Observed MW:	130kDa

Synonyms:

SPN, CD43, GALGP, GPL115, LSN

Background:

The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response.

UniProt Protein Function:	sialophorin: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX. Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
UniProt Protein Details:	Protein type:Cell surface; Membrane protein, integral Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; plasma membrane; basement membrane; uropod; external side of plasma membrane Molecular Function:protein binding; transmembrane receptor activity Biological Process: positive regulation of tumor necrosis factor biosynthetic process; regulation of defense response to virus; establishment and/or maintenance of cell polarity; negative regulation of cell adhesion; chemotaxis; signal transduction; negative thymic T cell selection; negative regulation of T cell proliferation; cell surface receptor linked signal transduction; response to protozoan; T cell costimulation; defense response to bacterium; cellular defense response; positive regulation of T cell proliferation; immune response; positive regulation of protein amino acid phosphorylation; blood coagulation; negative regulation of type IV hypersensitivity; leukocyte migration
NCBI Summary:	The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]
UniProt Code:	P16150
NCBI GenInfo Identifier:	126213
NCBI Gene ID:	6693
NCBI Accession:	P16150.1
UniProt Secondary Accession:	P16150,A8K9B1,
UniProt Related Accession:	P16150
Molecular Weight:
NCBI Full Name:	Leukosialin
NCBI Synonym Full Names:	sialophorin
NCBI Official Symbol:	SPN
NCBI Official Synonym Symbols:	LSN; CD43; GALGP; GPL115
NCBI Protein Information:	leukosialin; galactoglycoprotein; leukocyte sialoglycoprotein; sialophorin (gpL115, leukosialin, CD43)
UniProt Protein Name:	Leukosialin
UniProt Synonym Protein Names:	Galactoglycoprotein; GALGP; Leukocyte sialoglycoprotein; Sialophorin; CD_antigen: CD43
Protein Family:	Leukosialin
UniProt Gene Name:	SPN
UniProt Entry Name:	LEUK_HUMAN

	Western blot analysis of extracts of THP-1 cells, using SPN antibody (CAB6412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human tonsil using SPN antibody (CAB6412) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using SPN antibody (CAB6412) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using SPN antibody (CAB6412) at dilution of 1:100 (40x lens).