The SPINK5/LEKTI Monoclonal Antibody (CAB20916) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in various applications including immunofluorescence and flow cytometry.SPINK5, also known as LEKTI, is a key player in skin barrier function and is associated with diseases such as Netherton syndrome, a rare genetic disorder characterized by skin abnormalities.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SPINK5/LEKTI Monoclonal Antibody
SKU:
CAB20916
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2888
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NS, NETS, LEKTI, LETKI, VAKTI, SPINK5 / LEKTI
Positive Sample:
HeLa, MCF7, RAW264.7, Mouse skin, Mouse stomach, Rat stomach
Cellular Localization:
Secreted.
Calculated MW:
121kDa
Observed MW:
37kDa/65kDa/145kDa
This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
11005
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SPINK5 / LEKTI Rabbit mAb (CAB20916) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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