Cell Cycle Antibodies 1

Anti-SPIN1 Antibody (CAB5853)

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SKU:
CAB5853
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-SPIN1 Antibody
Antibody SKU:CAB5853
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIN1 (NP_006708.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, SW480, 293T, Jurkat, HeLa, HepG2, SH-SY5Y, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIN1 (NP_006708.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MKTP FGKT PGQR SRAD AGHA GVSA NMMK KRTS HKKH RSSV GPSK PVSQ PRRN IVGC RIQH GWKE GNGP VTQW KGTV LDQV PVNP SLYL IKYD GFDC VYGL ELNK DERV SALE VLPD RVAT SRIS DAHL ADTM IGKA VEHM FETE DGSK DEWR GMVL ARAP VMNT WFYI TYEK DPVL YMYQ LLDD YKEG DLRI MPDS NDSP PAER EPGE VVDS LVGK QVEY AKED GSKR TGMV IHQV EAKP SVYF IKFD DDFH IYVY DLVK TS
Gene ID:10927
Uniprot:Q9Y657
Cellular Location:Nucleus, nucleolus
Calculated MW:29kDa
Observed MW:32kDa
Synonyms:SPIN1, SPIN, TDRD24
Background:
UniProt Protein Function:SPIN1: May play a role in cell-cycle regulation during the transition from gamete to embryo. Belongs to the SPIN/STSY family.
UniProt Protein Details:

Protein type:Nucleolus; DNA-binding

Chromosomal Location of Human Ortholog: 9q22.1

Cellular Component: nucleolus; spindle; nucleus

Molecular Function:protein binding; methylated histone residue binding

Biological Process: Wnt receptor signaling pathway; meiotic cell cycle; multicellular organismal development; gamete generation; chromatin modification; positive regulation of Wnt receptor signaling pathway; rRNA transcription

UniProt Code:Q9Y657
NCBI GenInfo Identifier:93141317
NCBI Gene ID:10927
NCBI Accession:Q9Y657.3
UniProt Secondary Accession:Q9Y657,Q7KZJ8, Q9GZT2, Q9H0N7, A8K0X6, B3KRQ4,
UniProt Related Accession:Q9Y657
Molecular Weight:Calculated MW: 29kDaObserved MW: 32kDa
NCBI Full Name:Spindlin-1
NCBI Synonym Full Names:spindlin 1
NCBI Official Symbol:SPIN1  
NCBI Official Synonym Symbols:SPIN  
NCBI Protein Information:spindlin-1; ovarian cancer-related protein; spindlin1
UniProt Protein Name:Spindlin-1
UniProt Synonym Protein Names:Ovarian cancer-related protein; Spindlin1
Protein Family:Spindlin
UniProt Gene Name:SPIN1  
UniProt Entry Name:SPIN1_HUMAN
Anti-SPIN1 Antibody (CAB5853)Western blot analysis of extracts of various cell lines, using SPIN1 antibody (CAB5853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SPIN1 Antibody (CAB5853)Immunohistochemistry of paraffin-embedded rat brain using SPIN1 antibody (CAB5853) at dilution of 1:100 (40x lens).
Anti-SPIN1 Antibody (CAB5853)Immunohistochemistry of paraffin-embedded mouse brain using SPIN1 antibody (CAB5853) at dilution of 1:100 (40x lens).
Anti-SPIN1 Antibody (CAB5853)Immunofluorescence analysis of A549 cells using SPIN1 antibody (CAB5853). Blue: DAPI for nuclear staining.
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