Antibody Name:	Anti-SOD2 Antibody [KO Validated]
Antibody SKU:	CAB1340
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-222 of human SOD2 (NP_000627.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HepG2, A-549, Mouse liver, Mouse brain, Mouse heart, Rat liver, Rat brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-222 of human SOD2 (NP_000627.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KHSL PDLP YDYG ALEP HINA QIMQ LHHS KHHA AYVN NLNV TEEK YQEA LAKG DVTA QIAL QPAL KFNG GGHI NHSI FWTN LSPN GGGE PKGE LLEA IKRD FGSF DKFK EKLT AASV GVQG SGWG WLGF NKER GHLQ IAAC PNQD PLQG TTGL IPLL GIDV WEHA YYLQ YKNV RPDY LKAI WNVI NWEN VTER YMAC KK
Gene ID:	6648
Uniprot:	P04179
Cellular Location:	Mitochondrion matrix
Calculated MW:	18kDa/19kDa/20kDa/24kDa
Observed MW:	25KDa/22KDa

Synonyms:

IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD, SOD2

Background:

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.

UniProt Protein Function:	SOD2: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the iron/manganese superoxide dismutase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; EC 1.15.1.1; Oxidoreductase Chromosomal Location of Human Ortholog: 6q25.3 Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane Molecular Function:identical protein binding; DNA binding; manganese ion binding; superoxide dismutase activity; oxygen binding Biological Process: oxygen homeostasis; positive regulation of nitric oxide biosynthetic process; removal of superoxide radicals; heart development; locomotory behavior; response to lipopolysaccharide; response to L-ascorbic acid; post-embryonic development; protein homotetramerization; negative regulation of cell proliferation; response to selenium ion; glutathione metabolic process; regulation of mitochondrial membrane potential; acetylcholine vasodilation involved in regulation of systemic arterial blood pressure; regulation of catalytic activity; regulation of blood pressure; response to gamma radiation; hemopoiesis; negative regulation of neuron apoptosis; response to axon injury; response to electrical stimulus; response to drug; erythrophore differentiation; release of cytochrome c from mitochondria; response to superoxide; superoxide metabolic process; liver development; negative regulation of fat cell differentiation; response to manganese ion; regulation of transcription from RNA polymerase II promoter; response to silicon dioxide; response to reactive oxygen species; iron ion homeostasis; response to hyperoxia; response to cadmium ion; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; age-dependent response to reactive oxygen species; detection of oxygen; response to zinc ion; negative regulation of fibroblast proliferation; response to hypoxia; neuron development; response to activity; response to cold; induction of apoptosis by oxidative stress; superoxide release; hydrogen peroxide biosynthetic process Disease: Microvascular Complications Of Diabetes, Susceptibility To, 6
NCBI Summary:	This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	P04179
NCBI GenInfo Identifier:	134665
NCBI Gene ID:	6648
NCBI Accession:	P04179.2
UniProt Secondary Accession:	P04179,P78434, Q16792, Q5TCM1, Q96EE6, Q9P2Z3, B2R7R1 B3KUK2, B4DL20, B4E3K9, E1P5A9,
UniProt Related Accession:	P04179
Molecular Weight:	222
NCBI Full Name:	Superoxide dismutase
NCBI Synonym Full Names:	superoxide dismutase 2, mitochondrial
NCBI Official Symbol:	SOD2
NCBI Official Synonym Symbols:	IPOB; MNSOD; MVCD6
NCBI Protein Information:	superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; indophenoloxidase B; Mn superoxide dismutase; mangano-superoxide dismutase; manganese-containing superoxide dismutase
UniProt Protein Name:	Superoxide dismutase [Mn], mitochondrial
Protein Family:	Superoxide dismutase
UniProt Gene Name:	SOD2
UniProt Entry Name:	SODM_HUMAN

	Western blot analysis of extracts of various cell lines, using SOD2 antibody (CAB1340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts from normal (control) and SOD2 Rabbit pAb knockout (KO) 293T cells, using SOD2 Rabbit pAb antibody (CAB1340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded rat testis using SOD2 Rabbit pAb (CAB1340) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using SOD2 Rabbit pAb (CAB1340) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SOD2 Rabbit pAb (CAB1340) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-SOD2 Antibody (CAB19576)	Human SOD2 / MnSOD ELISA Kit
SOD2 Antibody (PACO02240)	Human Superoxide dismutase [Mn], mitochondrial (SOD2) ELISA Kit
SOD2 Antibody (PACO07027)
SOD2 Antibody (PACO07035)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)