Antibody Name:	Anti-SOCS5 Antibody
Antibody SKU:	CAB7952
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOCS5 (NP_054730.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	A-431, HeLa, Raji, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOCS5 (NP_054730.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDKV GKMW NNFK YRCQ NLFG HEGG SRSE NVDM NSNR CLSV KEKN ISIG DSTP QQQS SPLR ENIA LQLG LSPS KNSS RRNQ NCAT EIPQ IVEI SIEK DNDS CVTP GTRL ARRD SYSR HAPW GGKK KHSC STKT QSSL DADK
Gene ID:	9655
Uniprot:	O75159
Cellular Location:
Calculated MW:	61kDa
Observed MW:	70kDa

Synonyms:

SOCS5, CIS6, CISH6, Cish5, SOCS-5

Background:

The protein encoded by this gene contains a SH2 domain and a SOCS BOX domain. The protein thus belongs to the suppressor of cytokine signaling (SOCS) family, also known as STAT-induced STAT inhibitor (SSI) protein family. SOCS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The specific function of this protein has not yet been determined. Two alternatively spliced transcript variants encoding an identical protein have been reported.

UniProt Protein Function:	SOCS5: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits for instance EGF signaling by mediating the degradation of the EGF receptor/EGFR. Involved in the regulation of T-helper cell differentiation by inhibiting of the IL4 signaling pathway which promotes differentiation into the Th2 phenotype. Can also partially inhibit IL6 and LIF signaling.
UniProt Protein Details:	Protein type:Inhibitor Chromosomal Location of Human Ortholog: 2p21 Cellular Component: cytoplasm Molecular Function:protein binding; protein kinase inhibitor activity; receptor tyrosine kinase binding; epidermal growth factor receptor binding Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor activity; negative regulation of T-helper 2 cell differentiation; regulation of growth; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; cytokine and chemokine mediated signaling pathway; protein ubiquitination; cell growth; positive regulation of T-helper 1 cell differentiation; JAK-STAT cascade; negative regulation of JAK-STAT cascade; negative regulation of signal transduction
NCBI Summary:	The protein encoded by this gene contains a SH2 domain and a SOCS BOX domain. The protein thus belongs to the suppressor of cytokine signaling (SOCS) family, also known as STAT-induced STAT inhibitor (SSI) protein family. SOCS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The specific function of this protein has not yet been determined. Two alternatively spliced transcript variants encoding an identical protein have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	O75159
NCBI GenInfo Identifier:	7662240
NCBI Gene ID:	9655
NCBI Accession:	NP_054730
UniProt Secondary Accession:	O75159,Q53SD4, Q8IYZ4,
UniProt Related Accession:	O75159
Molecular Weight:	61,246 Da
NCBI Full Name:	suppressor of cytokine signaling 5
NCBI Synonym Full Names:	suppressor of cytokine signaling 5
NCBI Official Symbol:	SOCS5
NCBI Official Synonym Symbols:	CIS6; CISH6; Cish5; SOCS-5
NCBI Protein Information:	suppressor of cytokine signaling 5
UniProt Protein Name:	Suppressor of cytokine signaling 5
UniProt Synonym Protein Names:	Cytokine-inducible SH2 protein 6; CIS-6; Cytokine-inducible SH2-containing protein 5
Protein Family:	Suppressor of cytokine signaling
UniProt Gene Name:	SOCS5
UniProt Entry Name:	SOCS5_HUMAN

	Western blot analysis of extracts of various cell lines, using SOCS5 antibody (CAB7952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human mammary cancer using SOCS5 antibody (CAB7952) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using SOCS5 antibody (CAB7952) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using SOCS5 antibody (CAB7952) at dilution of 1:100 (40x lens).