Developmental Biology

Anti-SOCS2 Antibody (CAB17035)

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SKU:
CAB17035
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-SOCS2 Antibody
Antibody SKU:CAB17035
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human SOCS2 (NP_001257400.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human
Positive Samples:A-549, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human SOCS2 (NP_001257400.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTLR CLEP SGNG GEGT RSQW GTAG SAEE PSPQ AARL AKAL RELG QTGW YWGS MTVN EAKE KLKE APEG TFLI RDSS HSDY LLTI SVKT SAGP TNLR IEYQ DGKF RLDS IICV KSKL KQFD SVVH LIDY YVQM CKDK RTGP EAPR NGTV HLYL TKPL YTSA PSLQ HLCR LTIN KCTG AIWG LPLP TRLK DYLE EYKF QV
Gene ID:8835
Uniprot:O14508
Cellular Location:
Calculated MW:22kDa
Observed MW:22kDa
Synonyms:SOCS2, CIS2, Cish2, SOCS-2, SSI-2, SSI2, STATI2
Background:This gene encodes a member of the suppressor of cytokine signaling (SOCS) family. SOCS family members are cytokine-inducible negative regulators of cytokine receptor signaling via the Janus kinase/signal transducer and activation of transcription pathway (the JAK/STAT pathway). SOCS family proteins interact with major molecules of signaling complexes to block further signal transduction, in part, by proteasomal depletion of receptors or signal-transducing proteins via ubiquitination. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10, interferon (IFN)-gamma and by cytokine receptors such as growth horomone receptor. The protein encoded by this gene interacts with the cytoplasmic domain of insulin-like growth factor-1 receptor (IGF1R) and is thought to be involved in the regulation of IGF1R mediated cell signaling. This gene has pseudogenes on chromosomes 20 and 22. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:SOCS2: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin- protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Interacts with IGF1 receptor, prolactin receptor and growth hormone (GH) receptor. Associates with the Elongin BC complex. By a subset of cytokines, including EPO/erythropoietin and CSF2/GM-CSF. High expression in heart, placenta, lung, kidney and prostate.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 12q

Cellular Component: cytoplasm; cytosol

Molecular Function:insulin-like growth factor receptor binding; protein binding; growth hormone receptor binding; SH3/SH2 adaptor activity; protein kinase inhibitor activity; JAK pathway signal transduction adaptor activity

Biological Process: positive regulation of signal transduction; cytokine and chemokine mediated signaling pathway; negative regulation of insulin receptor signaling pathway; protein ubiquitination; regulation of signal transduction; JAK-STAT cascade; response to estradiol stimulus; cellular response to hormone stimulus; negative regulation of protein kinase activity; regulation of cell growth; negative regulation of JAK-STAT cascade; aging; negative regulation of apoptosis

NCBI Summary:This gene encodes a member of the suppressor of cytokine signaling (SOCS) family. SOCS family members are cytokine-inducible negative regulators of cytokine receptor signaling via the Janus kinase/signal transducer and activation of transcription pathway (the JAK/STAT pathway). SOCS family proteins interact with major molecules of signaling complexes to block further signal transduction, in part, by proteasomal depletion of receptors or signal-transducing proteins via ubiquitination. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10, interferon (IFN)-gamma and by cytokine receptors such as growth horomone receptor. The protein encoded by this gene interacts with the cytoplasmic domain of insulin-like growth factor-1 receptor (IGF1R) and is thought to be involved in the regulation of IGF1R mediated cell signaling. This gene has pseudogenes on chromosomes 20 and 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
UniProt Code:O14508
NCBI GenInfo Identifier:20178092
NCBI Gene ID:8835
NCBI Accession:O14508.1
UniProt Secondary Accession:O14508,O14542, O95102, Q9UKS5, A8K3D1,
UniProt Related Accession:O14508
Molecular Weight:198
NCBI Full Name:Suppressor of cytokine signaling 2
NCBI Synonym Full Names:suppressor of cytokine signaling 2
NCBI Official Symbol:SOCS2  
NCBI Official Synonym Symbols:CIS2; SSI2; Cish2; SSI-2; SOCS-2; STATI2  
NCBI Protein Information:suppressor of cytokine signaling 2; CIS-2; STAT induced STAT inhibitor-2; STAT-induced STAT inhibitor 2; STAT-induced STAT inhibitor-2; cytokine-inducible SH2 protein 2; suppressor of cytokine signaling-2
UniProt Protein Name:Suppressor of cytokine signaling 2
UniProt Synonym Protein Names:Cytokine-inducible SH2 protein 2; CIS-2; STAT-induced STAT inhibitor 2; SSI-2
Protein Family:Suppressor of cytokine signaling
UniProt Gene Name:SOCS2  
UniProt Entry Name:SOCS2_HUMAN
Anti-SOCS2 Antibody (CAB17035)Western blot analysis of extracts of various cell lines, using SOCS2 Rabbit pAb (CAB17035) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-SOCS2 Antibody (CAB17035)Immunofluorescence analysis of U-2 OS cells using SOCS2 Polyclonal Antibody (CAB17035) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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