Antibody Name:	Anti-SNIP1 Antibody
Antibody SKU:	CAB16747
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human SNIP1.

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	BxPC-3

Immunogen:	Recombinant protein of human SNIP1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	79753
Uniprot:	Q8TAD8
Cellular Location:
Calculated MW:	45kDa
Observed MW:	46kDa

Synonyms:

SNIP1, PML1, PMRED

Background:

This gene encodes a protein that contains a coiled-coil motif and C-terminal forkhead-associated (FHA) domain. The encoded protein functions as a transcriptional coactivator that increases c-Myc activity and inhibits transforming growth factor beta (TGF-beta) and nuclear factor kappa-B (NF-kB) signaling. The encoded protein also regulates the stability of cyclin D1 mRNA, and may play a role in cell proliferation and cancer progression. Mutations in this gene are a cause of psychomotor retardation, epilepsy, and craniofacial dysmorphism (PMRED).

UniProt Protein Function:	SNIP1: Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis. Defects in SNIP1 are the cause of psychomotor retardation, epilepsy, and craniofacial dysmorphism (PMRED). A disease characterized by severe psychomotor retardation, intractable seizures, dysmorphic features, and a lumpy skull surface. Patients are hypotonic and have poor feeding in the neonatal period.
UniProt Protein Details:	Protein type:Inhibitor Chromosomal Location of Human Ortholog: 1p34.3 Cellular Component: nucleoplasm; cytoplasm Molecular Function:protein binding Biological Process: miRNA-mediated gene silencing, production of miRNAs; I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent Disease: Psychomotor Retardation, Epilepsy, And Craniofacial Dysmorphism
NCBI Summary:	This gene encodes a protein that contains a coiled-coil motif and C-terminal forkhead-associated (FHA) domain. The encoded protein functions as a transcriptional coactivator that increases c-Myc activity and inhibits transforming growth factor beta (TGF-beta) and nuclear factor kappa-B (NF-kB) signaling. The encoded protein also regulates the stability of cyclin D1 mRNA, and may play a role in cell proliferation and cancer progression. Mutations in this gene are a cause of psychomotor retardation, epilepsy, and craniofacial dysmorphism (PMRED). [provided by RefSeq, Mar 2012]
UniProt Code:	Q8TAD8
NCBI GenInfo Identifier:	48428655
NCBI Gene ID:	79753
NCBI Accession:	Q8TAD8.1
UniProt Related Accession:	Q8TAD8
Molecular Weight:	46 kDa
NCBI Full Name:	Smad nuclear-interacting protein 1
NCBI Synonym Full Names:	Smad nuclear interacting protein 1
NCBI Official Symbol:	SNIP1
NCBI Official Synonym Symbols:	PML1; PMRED
NCBI Protein Information:	smad nuclear-interacting protein 1
UniProt Protein Name:	Smad nuclear-interacting protein 1
UniProt Synonym Protein Names:	FHA domain-containing protein SNIP1
Protein Family:	Smad nuclear interacting protein
UniProt Gene Name:	SNIP1
UniProt Entry Name:	SNIP1_HUMAN

	Western blot analysis of extracts of BxPC-3 cells, using SNIP1 Rabbit pAb (CAB16747) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using SNIP1 antibody (CAB16747) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using SNIP1 antibody (CAB16747) at dilution of 1:100. Blue: DAPI for nuclear staining.