Anti-SMURF2 Antibody (CAB2278)

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SKU:
CAB2278
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
SMURF2
Synonyms:
E3 ubiquitin-protein ligase SMURF2

Description

Product Name:SMURF2 Rabbit mAb
Product Code:CAB2278
Size:20uL, 50uL, 100uL
Synonyms:SMURF2, E3 ubiquitin-protein ligase SMURF2
Applications:WB, IHC, IF, IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human SMURF2
Applications:WB, IHC, IF, IP
Recommended Dilutions:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:U2OS, MCF7, SH-SY5Y, Mouse lung, Mouse testis, Mouse brain
Immunogen:A synthesized peptide derived from human SMURF2
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:64750
Uniprot:Q9HAU4
Cellular Location:Cell membrane, Cytoplasm, Membrane raft, Nucleus
Calculated MW:86kDa
Observed MW:80kDa
UniProt Protein Function:SMURF2: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
UniProt Protein Details:

Protein type:EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 17q23.3-q24.1

Cellular Component: cytosol; nuclear speck; nucleoplasm; ubiquitin ligase complex

Molecular Function:identical protein binding; protein binding; SMAD binding; ubiquitin-protein ligase activity

Biological Process: BMP signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; protein deubiquitination; protein polyubiquitination; ubiquitin-dependent protein catabolic process; ubiquitin-dependent SMAD protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway

UniProt Code:Q9HAU4
NCBI GenInfo Identifier:17865624
NCBI Gene ID:64750
NCBI Accession:Q9HAU4.1
UniProt Secondary Accession:Q9HAU4,Q52LL1, Q9H260,
UniProt Related Accession:Q9HAU4
Molecular Weight:86kDa
NCBI Full Name:E3 ubiquitin-protein ligase SMURF2
NCBI Synonym Full Names:SMAD specific E3 ubiquitin protein ligase 2
NCBI Official Symbol:SMURF2  
NCBI Protein Information:E3 ubiquitin-protein ligase SMURF2
UniProt Protein Name:E3 ubiquitin-protein ligase SMURF2
UniProt Synonym Protein Names:HECT-type E3 ubiquitin transferase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:SMURF2  
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