Signal Transduction Antibodies 1
Anti-SMURF2 Antibody (CAB10592)
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- SKU:
- CAB10592
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-SMURF2 Antibody |
| Antibody SKU: | CAB10592 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 150-250 of human SMURF2 (NP_073576.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | SW480 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 150-250 of human SMURF2 (NP_073576.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DCSR LFDN DLPD GWEE RRTA SGRI QYLN HITR TTQW ERPT RPAS EYSS PGRP LSCF VDEN TPIS GTNG ATCG QSSD PRLA ERRV RSQR HRNY MSRT HLHT P |
| Gene ID: | 64750 |
| Uniprot: | Q9HAU4 |
| Cellular Location: | Cell membrane, Cytoplasm, Membrane raft, Nucleus |
| Calculated MW: | 86kDa |
| Observed MW: | 86kDa |
| Synonyms: | SMURF2 |
| Background: |
| UniProt Protein Function: | SMURF2: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 17q23.3-q24.1 Cellular Component: cytosol; nuclear speck; nucleoplasm; ubiquitin ligase complex Molecular Function:identical protein binding; protein binding; SMAD binding; ubiquitin-protein ligase activity Biological Process: BMP signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; protein deubiquitination; protein polyubiquitination; ubiquitin-dependent protein catabolic process; ubiquitin-dependent SMAD protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway |
| UniProt Code: | Q9HAU4 |
| NCBI GenInfo Identifier: | 17865624 |
| NCBI Gene ID: | 64750 |
| NCBI Accession: | Q9HAU4.1 |
| UniProt Secondary Accession: | Q9HAU4,Q52LL1, Q9H260, |
| UniProt Related Accession: | Q9HAU4 |
| Molecular Weight: | 86kDa |
| NCBI Full Name: | E3 ubiquitin-protein ligase SMURF2 |
| NCBI Synonym Full Names: | SMAD specific E3 ubiquitin protein ligase 2 |
| NCBI Official Symbol: | SMURF2 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase SMURF2 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase SMURF2 |
| UniProt Synonym Protein Names: | HECT-type E3 ubiquitin transferase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | SMURF2 |
 | Western blot analysis of extracts of SW480 cells, using SMURF2 antibody (CAB10592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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