Neuroscience
Anti-SMARCD1 Antibody (CAB6310)
- SKU:
- CAB6310
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Neuroscience
Description
| Antibody Name: | Anti-SMARCD1 Antibody |
| Antibody SKU: | CAB6310 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 216-515 of human SMARCD1 (NP_003067.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Rat brain, Mouse thymus, Mouse heart, Mouse testis, Mouse brain, Jurkat, SW480, BT-474, SW620 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 216-515 of human SMARCD1 (NP_003067.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LEDS ALSK YDAT KQKR KFSS FFKS LVIE LDKD LYGP DNHL VEWH RTAT TQET DGFQ VKRP GDVN VRCT VLLM LDYQ PPQF KLDP RLAR LLGI HTQT RPVI IQAL WQYI KTHK LQDP HERE FVIC DKYL QQIF ESQR MKFS EIPQ RLHA LLMP PEPI IINH VISV DPND QKKT ACYD IDVE VDDT LKTQ MNSF LLST ASQQ EIAT LDNK IHET IETI NQLK TQRE FMLS FARD PQGF INDW LQSQ CRDL KTMT DVVG NPEE ERRA EFYF QPWA QEAV CRYF YSKV QQRR QELE QALG IRNT |
| Gene ID: | 6602 |
| Uniprot: | Q96GM5 |
| Cellular Location: | Nucleus |
| Calculated MW: | 53kDa/58kDa |
| Observed MW: | 70kDa |
| Synonyms: | SMARCD1, BAF60A, CRACD1, Rsc6p |
| Background: | The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | SMARCD1: Involved in chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer. Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the SMARCD family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 12q13-q14 Cellular Component: SWI/SNF complex Molecular Function:protein binding; transcription coactivator activity; protein complex scaffold Biological Process: regulation of transcription from RNA polymerase II promoter; chromatin remodeling; nervous system development; nucleosome disassembly; chromatin-mediated maintenance of transcription |
| NCBI Summary: | The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96GM5 |
| NCBI GenInfo Identifier: | 238054318 |
| NCBI Gene ID: | 6602 |
| NCBI Accession: | Q96GM5.2 |
| UniProt Related Accession: | Q96GM5 |
| Molecular Weight: | |
| NCBI Full Name: | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 |
| NCBI Synonym Full Names: | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 |
| NCBI Official Symbol: | SMARCD1 |
| NCBI Official Synonym Symbols: | Rsc6p; BAF60A; CRACD1 |
| NCBI Protein Information: | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 |
| UniProt Protein Name: | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 |
| UniProt Synonym Protein Names: | 60 kDa BRG-1/Brm-associated factor subunit A; BRG1-associated factor 60A; BAF60A; SWI/SNF complex 60 kDa subunit |
| UniProt Gene Name: | SMARCD1 |
| UniProt Entry Name: | SMRD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SMARCD1 antibody (CAB6310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
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