Neuroscience

Anti-SMARCC2 Antibody (CAB1967)

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SKU:
CAB1967
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Neuroscience

Description

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Antibody Name:Anti-SMARCC2 Antibody
Antibody SKU:CAB1967
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SMARCC2 (NP_620706.1).
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, Mouse spinal cord
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SMARCC2 (NP_620706.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PKLL GKLK DIIK RHQG TVTE DKNN ASHV VYPV PGNL EEEE WVRP VMKR DKQV LLHW GYYP DSYD TWIP ASEI EASV EDAP TPEK PRKV HAKW ILDT DTFN E
Gene ID:6601
Uniprot:Q8TAQ2
Cellular Location:Nucleus
Calculated MW:124kDa/126kDa/132kDa
Observed MW:171kDa
Synonyms:SMARCC2, BAF170, CRACC2, Rsc8
Background:The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:11018012). Can stimulate the ATPase activity of the catalytic subunit of these complexes (PubMed:10078207). May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells (PubMed:12192000). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation.
NCBI Summary:The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q8TAQ2
NCBI GenInfo Identifier:57012959
NCBI Gene ID:6601
NCBI Accession:Q8TAQ2.1
UniProt Secondary Accession:Q8TAQ2,Q59GV3, Q92923, Q96E12, Q96GY4, F8VTJ5,
UniProt Related Accession:Q8TAQ2
Molecular Weight:181 kDa
NCBI Full Name:SWI/SNF complex subunit SMARCC2
NCBI Synonym Full Names:SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 2
NCBI Official Symbol:SMARCC2  
NCBI Official Synonym Symbols:Rsc8; BAF170; CRACC2  
NCBI Protein Information:SWI/SNF complex subunit SMARCC2
UniProt Protein Name:SWI/SNF complex subunit SMARCC2
UniProt Synonym Protein Names:BRG1-associated factor 170; BAF170; SWI/SNF complex 170 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2
Protein Family:SWI/SNF complex
UniProt Gene Name:SMARCC2  
Anti-SMARCC2 Antibody (CAB1967)Western blot analysis of extracts of various cell lines, using SMARCC2 antibody (CAB1967) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
Anti-SMARCC2 Antibody (CAB1967)Immunohistochemistry of paraffin-embedded rat kidney using SMARCC2 antibody (CAB1967) at dilution of 1:100 (40x lens).
Anti-SMARCC2 Antibody (CAB1967)Immunohistochemistry of paraffin-embedded human prostate cancer using SMARCC2 antibody (CAB1967) at dilution of 1:100 (40x lens).
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