Antibody Name:	Anti-SMARCB1 Antibody [KO Validated]
Antibody SKU:	CAB5767
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 77-376 of human SMARCB1 (NP_001007469.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, THP-1, HepG2, HeLa, Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 77-376 of human SMARCB1 (NP_001007469.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TSVT LLKA SEVE EILD GNDE KYKA VSIS TEPP TYLR EQKA KRNS QWVP TLPN SSHH LDAV PCST TINR NRMG RDKK RTFP LCFD DHDP AVIH ENAS QPEV LVPI RLDM EIDG QKLR DAFT WNMN EKLM TPEM FSEI LCDD LDLN PLTF VPAI ASAI RQQI ESYP TDSI LEDQ SDQR VIIK LNIH VGNI SLVD QFEW DMSE KENS PEKF ALKL CSEL GLGG EFVT TIAY SIRG QLSW HQKT YAFS ENPL PTVE IAIR NTGD ADQW CPLL ETLT DAEM EKKI RDQD RNTR RMRR LANT APAW
Gene ID:	6598
Uniprot:	Q12824
Cellular Location:	Nucleus
Calculated MW:	43kDa/44kDa
Observed MW:	44KDa

Synonyms:

SMARCB1, BAF47, CSS3, INI1, MRD15, PPP1R144, RDT, RTPS1, SNF5, SNF5L1, SWNTS1, Sfh1p, Snr1, hSNFS

Background:

The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	SMARCB1: a core component of the BAF (hSWI/SNF) complex. This family of complexes remodels chromatin structures, enabling transcription factors to gain access to DNA. They play important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling is due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4. Involved in activation of CSF1 promoter. Belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain BAF53A and PHF10, are exchanged for homologous alternative BAF53B and BAF45B or BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene. Component of the BAF (hSWI/SNF) complex, which includes at least actin (ACTB), ARID1A, ARID1B, SMARCA2, SMARCA4, BAF53A, BAF53B, SMARCE1 SMARCC1, SMARCC2, SMARCB1, and one or more of SMARCD1, SMARCD2, or SMARCD3. In muscle cells, the BAF complex also contains DPF3. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Binds to double-stranded DNA. Interacts with EphB4 and MAEL. Interacts with GADD34. Binds tightly to the human immunodeficiency virus-type 1 (HIV-1) integrase in vitro and stimulates its DNA-joining activity. Interacts with human papillomavirus 18 E1 protein to stimulates its viral replication. Belongs to the SNF5 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Tumor suppressor; Transcription, coactivator/corepressor; Cell cycle regulation Chromosomal Location of Human Ortholog: 22q11.23\|22q11 Cellular Component: nuclear chromatin; nucleolus; nucleoplasm; nucleus; protein complex; SWI/SNF complex Molecular Function:DNA binding; nucleosomal DNA binding; p53 binding; protein binding; Tat protein binding; transcription coactivator activity Biological Process: ATP-dependent chromatin remodeling; cell differentiation; chromatin remodeling; DNA integration; DNA repair; negative regulation of cell proliferation; nucleosome disassembly; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter Disease: Mental Retardation, Autosomal Dominant 15; Rhabdoid Tumor Predisposition Syndrome 1; Schwannomatosis 1
NCBI Summary:	The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]
UniProt Code:	Q12824
NCBI GenInfo Identifier:	51338799
NCBI Gene ID:	6598
NCBI Accession:	Q12824.2
UniProt Secondary Accession:	Q12824,O75784, O95474, Q17S11, Q38GA1, Q76N08, Q9UBH2
UniProt Related Accession:	Q12824,AAB34227
Molecular Weight:	43,158 Da
NCBI Full Name:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
NCBI Synonym Full Names:	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
NCBI Official Symbol:	SMARCB1
NCBI Official Synonym Symbols:	RDT; CSS3; INI1; SNF5; Snr1; BAF47; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144
NCBI Protein Information:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
UniProt Protein Name:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
UniProt Synonym Protein Names:	BRG1-associated factor 47; BAF47; Integrase interactor 1 protein; SNF5 homolog; hSNF5
Protein Family:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily
UniProt Gene Name:	SMARCB1
UniProt Entry Name:	SNF5_HUMAN

	Western blot analysis of extracts of various cell lines, using SMARCB1 antibody (CAB5767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat Intestine using SMARCB1 antibody (CAB5767) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse osteosarcoma using SMARCB1 antibody (CAB5767) at dilution of 1:200 (40x lens).