Metabolism Antibodies 1

Anti-SLC27A2 Antibody (CAB1077)

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SKU:
CAB1077
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-SLC27A2 Antibody
Antibody SKU:CAB1077
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human SLC27A2 (NP_001153101.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human SLC27A2 (NP_001153101.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IGYF LKVA AVGR RVRS YGKR RPAR TILR AFLE KARQ TPHK PFLL FRDE TLTY AQVD RRSN QVAR ALHD HLGL RQGD CVAL LMGN EPAY VWLW LGLV KLGC AMAC LNYN IRAK SLLH CFQC CGAK VLLV SPEL QAAV EEIL PSLK KDDV SIYY VSRT SNTD GIDS FLDK VDE
Gene ID:11001
Uniprot:O14975
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein, Peroxisome membrane
Calculated MW:64kDa/70kDa
Observed MW:70kDa
Synonyms:SLC27A2, ACSVL1, FACVL1, FATP2, HsT17226, VLACS, VLCS, hFACVL1
Background:The protein encoded by this gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme activates long-chain, branched-chain and very-long-chain fatty acids containing 22 or more carbons to their CoA derivatives. It is expressed primarily in liver and kidney, and is present in both endoplasmic reticulum and peroxisomes, but not in mitochondria. Its decreased peroxisomal enzyme activity is in part responsible for the biochemical pathology in X-linked adrenoleukodystrophy. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SLC27A2: Acyl-CoA synthetase probably involved in bile acid metabolism. Proposed to activate C27 precurors of bile acids to their CoA thioesters derivatives before side chain cleavage via peroxisomal beta-oxidation occurs. In vitro, activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Does not utilize C24 bile acids as substrates. In vitro, also activates long- and branched-chain fatty acids and may have additional roles in fatty acid metabolism. May be involved in translocation of long-chain fatty acids (LFCA) across membranes. Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; EC 6.2.1.3; Membrane protein, integral; Ligase

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: peroxisomal membrane; integral to peroxisomal membrane; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum lumen; integral to endoplasmic reticulum membrane

Molecular Function:enzyme binding; fatty acid transporter activity; very-long-chain-fatty-acid-CoA ligase activity; phytanate-CoA ligase activity; ATP binding; receptor binding; long-chain-fatty-acid-CoA ligase activity

Biological Process: bile acid biosynthetic process; fatty acid beta-oxidation; very-long-chain fatty acid catabolic process; bile acid metabolic process; cellular lipid metabolic process; long-chain fatty acid metabolic process; fatty acid alpha-oxidation

NCBI Summary:The protein encoded by this gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme activates long-chain, branched-chain and very-long-chain fatty acids containing 22 or more carbons to their CoA derivatives. It is expressed primarily in liver and kidney, and is present in both endoplasmic reticulum and peroxisomes, but not in mitochondria. Its decreased peroxisomal enzyme activity is in part responsible for the biochemical pathology in X-linked adrenoleukodystrophy. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
UniProt Code:O14975
NCBI GenInfo Identifier:308153494
NCBI Gene ID:11001
NCBI Accession:O14975.2
UniProt Secondary Accession:O14975,Q53FY6, Q6PF09, A8K2J7,
UniProt Related Accession:O14975
Molecular Weight:620
NCBI Full Name:Very long-chain acyl-CoA synthetase
NCBI Synonym Full Names:solute carrier family 27 (fatty acid transporter), member 2
NCBI Official Symbol:SLC27A2  
NCBI Official Synonym Symbols:VLCS; FATP2; VLACS; ACSVL1; FACVL1; hFACVL1; HsT17226  
NCBI Protein Information:very long-chain acyl-CoA synthetase; FATP-2; THCA-CoA ligase; fatty acid transport protein 2; long-chain-fatty-acid--CoA ligase; solute carrier family 27 member 2; very-long-chain acyl-CoA synthetase; very long-chain-fatty-acid-CoA ligase; very long-chain
UniProt Protein Name:Very long-chain acyl-CoA synthetase
UniProt Synonym Protein Names:Fatty acid transport protein 2; FATP-2; Fatty-acid-coenzyme A ligase, very long-chain 1; Long-chain-fatty-acid--CoA ligase (EC:6.2.1.3); Solute carrier family 27 member 2; THCA-CoA ligase; Very long-chain-fatty-acid-CoA ligase
UniProt Gene Name:SLC27A2  
UniProt Entry Name:S27A2_HUMAN
Anti-SLC27A2 Antibody (CAB1077)Western blot analysis of extracts of HepG2 cells, using SLC27A2 antibody (CAB1077). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-SLC27A2 Antibody (CAB1077)Immunohistochemistry of paraffin-embedded human liver using SLC27A2 antibody (CAB1077) at dilution of 1:100 (40x lens).
Anti-SLC27A2 Antibody (CAB1077)Immunohistochemistry of paraffin-embedded human gastric cancer using SLC27A2 antibody (CAB1077) at dilution of 1:100 (40x lens).
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