Antibody Name:	Anti-SLC25A5 Antibody
Antibody SKU:	CAB15639
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SLC25A5 (NP_001143.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, LO2, Mouse brain, Mouse heart, Mouse kidney, Rat kidney, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SLC25A5 (NP_001143.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AERE FRGL GDCL VKIY KSDG IKGL YQGF NVSV QGII IYRA AYFG IYDT AKGM LPDP KNTH IVIS WMIA QTVT AVAG LTSY PFDT VRRR MMMQ SGRK GTDI M
Gene ID:	292
Uniprot:	P05141
Cellular Location:	Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:	32kDa
Observed MW:	33kDa

Synonyms:

SLC25A5, 2F1, AAC2, ANT2, T2, T3

Background:

This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Suppressed expression of this gene has been shown to induce apoptosis and inhibit tumor growth. The human genome contains several non-transcribed pseudogenes of this gene.

UniProt Protein Function:	SLC25A5: Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation. Belongs to the mitochondrial carrier family.
UniProt Protein Details:	Protein type:Transporter; Mitochondrial; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: Xq24 Cellular Component: integral to plasma membrane; membrane; mitochondrial inner membrane; mitochondrion; myelin sheath; nucleus Molecular Function:adenine transmembrane transporter activity; protein binding; structural constituent of ribosome; ubiquitin protein ligase binding Biological Process: adenine transport; chromosome segregation; positive regulation of cell proliferation; regulation of insulin secretion; translation; transmembrane transport; transport; viral reproduction
NCBI Summary:	This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Suppressed expression of this gene has been shown to induce apoptosis and inhibit tumor growth. The human genome contains several non-transcribed pseudogenes of this gene.[provided by RefSeq, Jun 2013]
UniProt Code:	P05141
NCBI GenInfo Identifier:	317373597
NCBI Gene ID:	292
NCBI Accession:	P05141.7
UniProt Secondary Accession:	P05141,O43350, B2RCV1,
UniProt Related Accession:	P05141
Molecular Weight:	33-36 kDa
NCBI Full Name:	ADP/ATP translocase 2
NCBI Synonym Full Names:	solute carrier family 25 member 5
NCBI Official Symbol:	SLC25A5
NCBI Official Synonym Symbols:	T2; T3; 2F1; AAC2; ANT2
NCBI Protein Information:	ADP/ATP translocase 2
UniProt Protein Name:	ADP/ATP translocase 2
UniProt Synonym Protein Names:	ADP,ATP carrier protein 2; ADP,ATP carrier protein, fibroblast isoform; Adenine nucleotide translocator 2; ANT 2
Protein Family:	ADP/ATP translocase
UniProt Gene Name:	SLC25A5
UniProt Entry Name:	ADT2_HUMAN

	Western blot analysis of extracts of various cell lines, using SLC25A5 antibody (CAB15639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat heart using SLC25A5 antibody (CAB15639) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using SLC25A5 antibody (CAB15639) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using SLC25A5 antibody (CAB15639) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.