KO Validated Antibodies 1

Anti-SLC25A22 Antibody (CAB17772)[KO Validated]

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SKU:
CAB17772
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SLC25A22 Antibody [KO Validated]
Antibody SKU:CAB17772
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 128-188 of human SLC25A22 (NP_078974.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HepG2, A-549, A-431, Mouse brain, Rat brain, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 128-188 of human SLC25A22 (NP_078974.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KIQL QDAG RIAA QRKI LAAQ GQLS AQGG AQPS VEAP AAPR PTAT QLTR DLLR SRGI AGLY K
Gene ID:79751
Uniprot:Q9H936
Cellular Location:
Calculated MW:
Observed MW:34kDa
Anti-SLC25A22 Antibody [KO Validated] (CAB17772)Western blot analysis of extracts of various cell lines, using SLC25A22 antibody (CAB17772) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-SLC25A22 Antibody [KO Validated] (CAB17772)Western blot analysis of extracts from normal (control) and SLC25A22 knockout (KO) 293T cells, using SLC25A22 antibody (CAB17772) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SLC25A22 Antibody [KO Validated] (CAB17772)Immunofluorescence analysis of C6 cells using SLC25A22 antibody (CAB17772) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-SLC25A22 Antibody [KO Validated] (CAB17772)Immunofluorescence analysis of L929 cells using SLC25A22 antibody (CAB17772) at dilution of 1:100. Blue: DAPI for nuclear staining.
Synonyms:EIEE3, GC-1, GC1, NET44, SLC25A22
Background:This gene encodes a mitochondrial glutamate carrier. Mutations in this gene are associated with early infantile epileptic encephalopathy. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jul 2010]
NCBI Summary:This gene encodes a mitochondrial glutamate carrier. Mutations in this gene are associated with early infantile epileptic encephalopathy. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jul 2010]
UniProt Code:Q9H936
NCBI GenInfo Identifier:13375983
NCBI Gene ID:79751
NCBI Accession:NP_078974
UniProt Related Accession:Q9H936
Molecular Weight:34kDa
NCBI Full Name:mitochondrial glutamate carrier 1
NCBI Synonym Full Names:solute carrier family 25 member 22
NCBI Official Symbol:SLC25A22  
NCBI Official Synonym Symbols:GC1; GC-1; EIEE3; NET44  
NCBI Protein Information:mitochondrial glutamate carrier 1
UniProt Protein Name:Mitochondrial glutamate carrier 1
UniProt Synonym Protein Names:Glutamate/H(+) symporter 1; Solute carrier family 25 member 22
Protein Family:Mitochondrial glutamate carrier
UniProt Gene Name:SLC25A22  
UniProt Entry Name:GHC1_HUMAN
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