Antibody Name:	Anti-SKAP2 Antibody
Antibody SKU:	CAB6435
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SKAP2 (NP_003921.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SKAP2 (NP_003921.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPNP SSTS SPYP LPEE IRNL LADV ETFV ADIL KGEN LSKK AKEK RESL IKKI KDVK SIYL QEFQ DKGD AEDG EEYD DPFA GPPD TISL ASER YDKD DEAP SDGA QFPP IAAQ DLPF VLKA
Gene ID:	8935
Uniprot:	O75563
Cellular Location:	Cytoplasm
Calculated MW:	41kDa
Observed MW:	50kDa

Synonyms:

SKAP2, PRAP, RA70, SAPS, SCAP2, SKAP-HOM, SKAP55R

Background:

The protein encoded by this gene shares homology with Src kinase-associated phosphoprotein 1, and is a substrate of Src family kinases. It is an adaptor protein that is thought to play an essential role in the Src signaling pathway, and in regulating proper activation of the immune system. This protein contains an amino terminal coiled-coil domain for self-dimerization, a plecskstrin homology (PH) domain required for interactions with lipids at the membrane, and a Src homology (SH3) domain at the carboxy terminus. Some reports indicate that this protein inhibits actin polymerization through interactions with actin assembly factors, and might negatively regulate the invasiveness of tumors by modulating actin assembly. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	RA70: an ubiquitous adapter protein thought to play a role in the src signaling pathway. Contains a coiled-coil domain, a pleckstrin homology domain, and a SH3 domain. The SH3 domain binds to the proline-rich domain of Pyk2, inhibiting alpha-synuclein tyrosine phosphorylation. Can be phosphorylated by Src family kinases but not by Pyk2. Functions as a downstream target for Pyk2 and plays a role in alpha-synuclein phosphorylation. Negatively regulates alpha-synuclein phosphorylation following cell stress.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 7p15.2 Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol Molecular Function:protein binding; SH3/SH2 adaptor activity Biological Process: negative regulation of cell proliferation; B cell activation; positive regulation of signal transduction; protein complex assembly; signal transduction
NCBI Summary:	The protein encoded by this gene shares homology with Src kinase-associated phosphoprotein 1, and is a substrate of Src family kinases. It is an adaptor protein that is thought to play an essential role in the Src signaling pathway, and in regulating proper activation of the immune system. This protein contains an amino terminal coiled-coil domain for self-dimerization, a plecskstrin homology (PH) domain required for interactions with lipids at the membrane, and a Src homology (SH3) domain at the carboxy terminus. Some reports indicate that this protein inhibits actin polymerization through interactions with actin assembly factors, and might negatively regulate the invasiveness of tumors by modulating actin assembly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]
UniProt Code:	O75563
NCBI GenInfo Identifier:	74735515
NCBI Gene ID:	8935
NCBI Accession:	O75563.1
UniProt Secondary Accession:	O75563,Q53GP6, Q75MK6, Q75MZ4, Q9UBZ3, Q9UED8, A4D173
UniProt Related Accession:	O75563
Molecular Weight:	359
NCBI Full Name:	Src kinase-associated phosphoprotein 2
NCBI Synonym Full Names:	src kinase associated phosphoprotein 2
NCBI Official Symbol:	SKAP2
NCBI Official Synonym Symbols:	PRAP; RA70; SAPS; SCAP2; SKAP55R; SKAP-HOM
NCBI Protein Information:	src kinase-associated phosphoprotein 2; SKAP-55HOM; SKAP55 homolog; Pyk2/RAFTK-associated protein; retinoic acid-induced protein 70; src family-associated phosphoprotein 2; Fyn-associated phosphoprotein SKAP55 homologue; src-associated adapter protein with PH and SH3 domains; src kinase-associated phosphoprotein of 55-related protein
UniProt Protein Name:	Src kinase-associated phosphoprotein 2
UniProt Synonym Protein Names:	Pyk2/RAFTK-associated protein; Retinoic acid-induced protein 70; SKAP55 homolog; SKAP-55HOM; SKAP-HOM; Src family-associated phosphoprotein 2; Src kinase-associated phosphoprotein 55-related protein; Src-associated adapter protein with PH and SH3 domains
Protein Family:	Src kinase-associated phosphoprotein
UniProt Gene Name:	SKAP2
UniProt Entry Name:	SKAP2_HUMAN

	Western blot analysis of extracts of mouse lung, using SKAP2 antibody (CAB6435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse stomach using SKAP2 antibody (CAB6435) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat lung using SKAP2 antibody (CAB6435) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using SKAP2 antibody (CAB6435) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using SKAP2 antibody (CAB6435). Blue: DAPI for nuclear staining.