The SIRT6 Monoclonal Antibody (CAB3591) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high specificity and sensitivity for human samples, making it ideal for use in Western blot applications. By binding to the SIRT6 protein, this antibody enables researchers to detect and analyze SIRT6 expression in various cell types, providing valuable insights into its functions in aging, metabolism, and disease processes.SIRT6, a member of the sirtuin family of proteins, plays a crucial role in regulating gene expression, DNA repair, and glucose metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SIRT6 Monoclonal Antibody
SKU:
CAB3591
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0808
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SIR2L6, hSIRT6, SIRT6
Positive Sample:
293T, HeLa, HCT 116
Cellular Localization:
Nucleus, Endoplasmic Reticulum.
Calculated MW:
36kDa/39kDa
Observed MW:
42kDa
This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
51548
RRID
AB_2863095
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KD Validated] SIRT6 Rabbit mAb (CAB3591) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Western blot analysis of lysates from wild type (WT) and SIRT6 knockdown (KD) 293T cells using SIRT6 Rabbit mAb (CAB3591) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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