Anti-SIRT3 Antibody [KO Validated] (CAB20805)

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SKU:
CAB20805

Description

Antibody Name:Anti-SIRT3 Antibody [KO Validated] (CAB20805)
Antibody SKU:CAB20805
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry, Immunoprecipitation
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300-399 of human SIRT3 (NP_036371.1).
Application:Western blotting, Immunohistochemistry, Immunoprecipitation
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa, HepG2, Mouse liver, Rat liver, Mouse kidney
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300-399 of human SIRT3 (NP_036371.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QRFL LHVV DFPM ADLL LILG TSLE VEPF ASLT EAVR SSVP RLLI NRDL VGPL AWHP RSRD VAQL GDVV HGVE SLVE LLGW TEEM RDLV QRET GKLD GPDK
Cellular Location:Mitochondrion matrix
Calculated MW:28kDa/43kDa
Observed MW:28kDa
Synonyms:SIRT3, SIR2L3, sirtuin 3
Background:This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug [KO Validated] SIRT3 antibody . Western blot was performed from the immunoprecipitate using [KO Validated] SIRT3 antibody at a dilition of 1:1000.Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug [KO Validated] SIRT3 antibody . Western blot was performed from the immunoprecipitate using [KO Validated] SIRT3 antibody at a dilition of 1:1000.
Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] SIRT3 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] SIRT3 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Western blot analysis of extracts of Mouse kidney, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Western blot analysis of extracts of Mouse kidney, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Western blot analysis of extracts from normal (control) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.Western blot analysis of extracts from normal (control) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.Western blot analysis of extracts of various cell lines, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
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Additional Information

Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Goat
Isotype:
IgG
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