KO Validated Antibodies 1

Anti-SIRT3 Antibody (CAB5718)[KO Validated]

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SKU:
CAB5718
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SIRT3 Antibody [KO Validated]
Antibody SKU:CAB5718
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:20 - 1:50 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HepG2, Mouse skeletal muscle, Mouse kidney, Rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QRFL LHVV DFPM ADLL LILG TSLE VEPF ASLT EAVR SSVP RLLI NRDL VGPL AWHP RSRD VAQL GDVV HGVE SLVE LLGW TEEM RDLV QRET GKLD GPDK
Gene ID:23410
Uniprot:Q9NTG7
Cellular Location:Mitochondrion matrix
Calculated MW:28kDa/43kDa
Observed MW:28KDa/44KDa
Synonyms:SIRT3, SIR2L3, sirtuin 3
Background:This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
UniProt Code:Q9NTG7
NCBI GenInfo Identifier:38258651
NCBI Gene ID:23410
NCBI Accession:Q9NTG7.2
UniProt Secondary Accession:Q9NTG7,Q9Y6E8, B7Z5U6,
UniProt Related Accession:Q9NTG7
Molecular Weight:399
NCBI Full Name:NAD-dependent protein deacetylase sirtuin-3, mitochondrial
NCBI Synonym Full Names:sirtuin 3
NCBI Official Symbol:SIRT3  
NCBI Official Synonym Symbols:SIR2L3  
NCBI Protein Information:NAD-dependent protein deacetylase sirtuin-3, mitochondrial; sir2-like 3; sirtuin type 3; SIR2-like protein 3; regulatory protein SIR2 homolog 3; NAD-dependent deacetylase sirtuin-3, mitochondrial; silent mating type information regulation 2, S.cerevisiae, homolog 3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
UniProt Protein Name:NAD-dependent protein deacetylase sirtuin-3, mitochondrial
UniProt Synonym Protein Names:Regulatory protein SIR2 homolog 3; SIR2-like protein 3
Protein Family:NAD-dependent protein deacetylase sirtuin
UniProt Gene Name:SIRT3  
UniProt Entry Name:SIR3_HUMAN
Anti-SIRT3 Antibody [KO Validated] (CAB5718)Western blot analysis of extracts of 293T cells, using [KO Validated] SIRT3 antibody (CAB5718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-SIRT3 Antibody [KO Validated] (CAB5718)Western blot analysis of extracts of various cell lines, using [KO Validated] SIRT3 antibody (CAB5718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-SIRT3 Antibody [KO Validated] (CAB5718)Western blot analysis of extracts of various cell lines, using [KO Validated] SIRT3 antibody (CAB5718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Anti-SIRT3 Antibody [KO Validated] (CAB5718)Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody (CAB5718) at dilution of 1:100 (40x lens).
Anti-SIRT3 Antibody [KO Validated] (CAB5718)Immunohistochemistry of paraffin-embedded human thyroid cancer using SIRT3 antibody (CAB5718) at dilution of 1:100 (40x lens).
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