Antibody Name:	Anti-SHMT2 Antibody [KO Validated]
Antibody SKU:	CAB1215
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T, SW620

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PSPF KHAD IVTT TTHK TLRG ARSG LIFY RKGV KAVD PKTG REIP YTFE DRIN FAVF PSLQ GGPH NHAI AAVA VALK QACT PMFR EYSL QVLK NARA MADA LLER GYSL VSGG TDNH LVLV DLRP KGLD GARA ERVL ELVS ITAN KNTC PGDR SAIT PGGL RLGA PALT SRQF REDD FRRV VDFI DEGV NIGL EVKS KTAK LQDF KSFL LKDS ETSQ RLAN LRQR VEQF ARAF PMPG FDEH
Gene ID:	6472
Uniprot:	P34897
Cellular Location:	Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix, mitochondrion nucleoid
Calculated MW:	53kDa/54kDa/55kDa
Observed MW:	56KDa

Synonyms:

SHMT2, GLYA, HEL-S-51e, SHMT

Background:

This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5, 10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SHMT2: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; Energy Metabolism - methane; Cofactor and Vitamin Metabolism - one carbon pool by folate; Mitochondrial; EC 2.1.2.1; Other Amino Acids Metabolism - cyanoamino acid Chromosomal Location of Human Ortholog: 12q12-q14 Cellular Component: microtubule cytoskeleton; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial intermembrane space Molecular Function:L-allo-threonine aldolase activity; identical protein binding; amino acid binding; glycine hydroxymethyltransferase activity; chromatin binding; pyridoxal phosphate binding Biological Process: L-serine biosynthetic process; positive regulation of cell proliferation; one-carbon compound metabolic process; glycine biosynthetic process from serine; protein homotetramerization
NCBI Summary:	This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Code:	P34897
NCBI GenInfo Identifier:	6226865
NCBI Gene ID:	6472
NCBI Accession:	P34897.3
UniProt Related Accession:	P34897
Molecular Weight:	~ 56kDa
NCBI Full Name:	Serine hydroxymethyltransferase, mitochondrial
NCBI Synonym Full Names:	serine hydroxymethyltransferase 2
NCBI Official Symbol:	SHMT2
NCBI Official Synonym Symbols:	GLYA; SHMT; HEL-S-51e
NCBI Protein Information:	serine hydroxymethyltransferase, mitochondrial
UniProt Protein Name:	Serine hydroxymethyltransferase, mitochondrial
UniProt Synonym Protein Names:	Glycine hydroxymethyltransferase; Serine methylase
UniProt Gene Name:	SHMT2
UniProt Entry Name:	GLYM_HUMAN

	Western blot analysis of extracts of various cell lines, using SHMT2 Rabbit pAb (CAB1215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Western blot analysis of extracts from normal (control) and SHMT2 Rabbit pAb knockout (KO) HeLa cells, using SHMT2 Rabbit pAb antibody (CAB1215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded rat spleen using SHMT2 antibody (CAB1215) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 antibody (CAB1215) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using SHMT2 antibody (CAB1215) at dilution of 1:100 (40x lens).