Developmental Biology

Anti-SFRP5 Antibody (CAB16734)

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SKU:
CAB16734
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-SFRP5 Antibody
Antibody SKU:CAB16734
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-317 of human SFRP5 (NP_003006.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-317 of human SFRP5 (NP_003006.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EEYD YYGW QAEP LHGR SYSK PPQC LDIP ADLP LCHT VGYK RMRL PNLL EHES LAEV KQQA SSWL PLLA KRCH SDTQ VFLC SLFA PVCL DRPI YPCR SLCE AVRA GCAP LMEA YGFP WPEM LHCH KFPL DNDL CIAV QFGH LPAT APPV TKIC AQCE MEHS ADGL MEQM CSSD FVVK MRIK EIKI ENGD RKLI GAQK KKKL LKPG PLKR KDTK RLVL HMKN GAGC PCPQ LDSL AGSF LVMG RKVD GQLL LMAV YRWD KKNK EMKF AVKF MFSY PCSL YYPF FYGA AEPH
Gene ID:6425
Uniprot:Q5T4F7
Cellular Location:
Calculated MW:35kDa
Observed MW:32kDa
Synonyms:SFRP5, SARP3
Background:Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.
UniProt Code:Q5T4F7
NCBI GenInfo Identifier:188528609
NCBI Gene ID:6425
NCBI Accession:NP_003006.2
UniProt Secondary Accession:Q5T4F7,O14780, Q86TH7,
UniProt Related Accession:Q5T4F7
Molecular Weight:35,563 Da
NCBI Full Name:secreted frizzled-related protein 5
NCBI Synonym Full Names:secreted frizzled-related protein 5
NCBI Official Symbol:SFRP5  
NCBI Official Synonym Symbols:SARP3  
NCBI Protein Information:secreted frizzled-related protein 5; FRP-1b; SARP-3; sFRP-5; frizzled-related protein 1b; secreted apoptosis related protein 3; secreted apoptosis-related protein 3
UniProt Protein Name:Secreted frizzled-related protein 5
UniProt Synonym Protein Names:Frizzled-related protein 1b; FRP-1b; Secreted apoptosis-related protein 3
Protein Family:Secreted frizzled-related protein
UniProt Gene Name:SFRP5  
UniProt Entry Name:SFRP5_HUMAN
Anti-SFRP5 Antibody (CAB16734)Western blot analysis of extracts of Mouse pancreas, using SFRP5 antibody (CAB16734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SFRP5 Antibody (CAB16734)Immunofluorescence analysis of mouse pancreas using SFRP5 Polyclonal Antibody (CAB16734) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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